ID   QL-EVE-DC-003; SV 0; linear; genomic DNA; STS; SYN; 482 BP.
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AC   IFD-25958-3;
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DT   27-JUL-2010
DT   18-MAY-2016
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DE   Qualitative PCR method for detection of carnation event 25958 (verified by the EURL GMFF in the context of Commission Implementing Decision (EU) 2015/692).
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KW   event_specific, EU-RL_GMFF_in-house_verified.
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OS   Dianthus caryophyllus (carnation) - event 25958 (IFD-25958-3)
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RN   [1]
RP   1-482
RA   Savini C., Brustio R., Bogni A., Mazzara M., Kreysa J.;
RT   "Report on the single-laboratory validation of a PCR-based Detection Method for Identification of FlorigeneTM IFD-25958-3 GM Carnation - Validation Report and Validated Method";
RL   Online Publication (2012).
RX   DOI=10.2788/76760
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RN   [2]
RP   1-482
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2016).
RX   PCR=QL-EVE-DC-003.pdf
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DR   GMOMETHODS; QL-TAX-DC-001;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..482
FT                   /standard_name="PCR 482 bp amplicon"
FT                   /note="Event-specific PCR";
FT                   /target="3' integration border region (IBR) between the insert of carnation event 25958 and the carnation host genome"
FT   primer_bind     1..24
FT                   /standard_name="Primer forward: RB Forward"
FT                   /note="TGGACCCTTGAGGAAACTGGTAGC"
FT                   /target="insert"
FT   primer_bind     complement(462..482)
FT                   /standard_name="Primer reverse: IFD-25958-3 Reverse"
FT                   /note="CTTGTACACCGCGTTATGCTC"
FT                   /target="3'-host genome" 
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SQ   Sequence 482 BP; 126 A; 89 C; 103 G; 164 T; 0 other;
     tggacccttg aggaaactgg tagcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       120
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       180
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       240
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       300
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       360
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       420
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngagcataac gcggtgtaca       480
     ag                                                                      482
//