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ID   QL-EVE-CP-001; SV 0; linear; genomic DNA; STD; SYN; 285 BP.
AC   ;
DT   26-JUN-2013
DT   01-DEC-2016
DE   Qualitative PCR method for detection of papaya event Huanong N 1 (Wei et al., 2016).
KW   event_specific.
OS   Carica papaya (papaya) - event Huanong No. 1 
RN   [1]
RP   1-285
RA   Wei J., Le H., Pan A., Xu J., Li F., Li X., Quan S., Guo J., Yang L.;
RT   "Collaborative trial for the validation of event-specific PCR detection methods of genetically modified papaya Huanong No. 1";
RL   Food Chemistry 194:20-25 (2016).
RX   DOI=10.1016/j.foodchem.2015.07.010.
RN   [2]
RP   1-285
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2016)
RX   PCR=QL-EVE-CP-001.pdf
FH   Key             Location/Qualifiers
FT   STS             1..285
FT                   /standard_name="PCR 285 bp amplicon"
FT                   /note="Event-specific PCR"
FT                   /target="5' integration border region (IBR) between the insert of papaya event Huanong No 1 and the papaya host genome"
FT   primer_bind     1..22
FT                   /standard_name="Primer forward: HN-F"
FT                   /note="ATTGACGAGTACAAGGAGACGC"
FT                   /target="5'-host genome"
FT   misc_feature    complement(137..228)
FT                   /note="nptII fragment"
FT   misc_feature    229..285
FT                   /note="Binary vector pBI121 fragment. Identical to
FT                   Genbank:AF485783 (2476-5826)"
FT   primer_bind     complement(265..285)
FT                   /standard_name="Primer reverse: HN-R"
FT                   /note="CTCCGCTCATGATCAGATTGT"
FT                   /target="insert"
SQ   Sequence 285 BP; 100 A; 59 C; 53 G; 73 T; 0 other;
     attgacgagt acaaggagac gccttttaat ttgtttaaat taaataaaaa cataaattat        60
     taagcgacgt aataataaac acgtcataaa ttttttcaaa aaataaaatt tcttttattt       120
     tctaaaaagt agttgattcg cccaatagca gccagtccct tcccgcttca gtgacaacgt       180
     cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagcctc aaacactgat       240
     agtttaaact gaaggcggga aacgacaatc tgatcatgag cggag                       285