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ID   QL-ELE-00-029; SV 0; linear; genomic DNA; STD; SYN; 204 BP.
AC   ;
DT   25-NOV-2010
DT   15-DEC-2017
DE   Qualitative LAMP method for detection of CP4 epsps gene  (Li et al., 2018).
KW   element_specific.
RN   [1]
RP   1-204
RA   Li R., Shi J., Liu B., Zhang D., Zhao X., Yang L.;
RT   "International collaborative ring trial of four gene-specific loop-mediated isothermal amplification assays in GMO analysis";
RL   Food Control 84:278-283 (2018).
RX   DOI=10.1016/j.foodcont.2017.08.012
RN   [2]
RP   1-204
RA   Wang C., Li R., Quan S., Shen P., Zhang D., Shi J., Yang L.;
RT   "GMO detection in food and feed through screening by visual loop-mediated isothermal amplification assays";
RL   Anal. and Bioanal. Chem.  407:4829-4834(2015).
RX   DOI=10.1007/s00216-015-8652-z
RN   [3]
RP   1-204
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2017).
RX   PCR=QL-ELE-00-029.pdf
FH   Key             Location/Qualifiers
FT   STS             1..204
FT                   /standard_name="LAMP 204 bp amplicon"
FT                   /note="element-specific LAMP assay"
FT                   /target="CP4 epsps gene from Agrobacterium tumefaciens type I and II"
FT   primer_bind     1..19
FT                   /standard_name="Primer forward: CP4 epsps F3"
FT                   /note="CTTGCGTGGACCAAAGACT"
FT                   /target="CP4 epsps"
FT   primer_bind     join(20..38,complement(60..81))
FT                   /standard_name="Primer: CP4 epsps FIP"
FT   primer_bind     20..38
FT                   /note="CCAACGCCAATCACCTACA"
FT                   /target="CP4 epsps F2"
FT   primer_bind     complement(39..59)
FT                   /standard_name="Primer: CP4 epsps loop F (complementary to the sequence between F1 and F2)"
FT                   /note="GAGCGGAAGCCATAGGTACCC"
FT   primer_bind     complement(60..81)
FT                   /note="AGCAGAACAGCGGACTTCACTT"
FT                   /target="CP4 epsps F1c (complementary to F1)"
FT   primer_bind     join(93..114,complement(152..171))
FT                   /standard_name="Primer: CP4 epsps BIP"
FT   primer_bind     93..114
FT                   /note="ACACCCCAGGTATCACCACTGT"
FT                   /target="CP4 epsps B1c (complementary to B1)"
FT   primer_bind     115..151
FT                   /standard_name="Primer: CP4 epsps loop B (complementary to the sequence between B1 and B2)"
FT   primer_bind     complement(152..171)
FT                   /note="GCACCAAAACCTTGAAGCAT"
FT                   /target="CP4 epsps B2"
FT   primer_bind     complement(186..204)
FT                   /standard_name="Primer reverse: CP4 epsps B3"
FT                   /note="ACACCGTCAGCATCAGTCT"
FT                   /target="CP4 epsps"
SQ   Sequence 204 BP; 48 A; 58 C; 46 G; 52 T; 0 other;
     cttgcgtgga ccaaagactc caacgccaat cacctacagg gtacctatgg cttccgctca        60
     agtgaagtcc gctgttctgc tnnnnnnnnn nnacacccca ggtatcacca ctgttatcga       120
     gccaatcatg actcgtgacc acactgaaaa gatgcttcaa ggttttggtg cnnnnnnnnn       180
     nnnnnagact gatgctgacg gtgt                                              204