ID   QL-ELE-00-028; SV 0; linear; genomic DNA; STD; SYN; 199 BP.
XX
AC   ;
XX
DT   07-APR-2008
DT   15-DEC-2017
XX
DE   Qualitative LAMP method for detection of phosphinothricin N-acetyltransferase (pat) gene (Li et al., 2018).
XX
KW   element_specific.
XX
RN   [1]
RP   1-199
RA   Li R., Shi J., Liu B., Zhang D., Zhao X., Yang L.;
RT   "International collaborative ring trial of four gene-specific loop-mediated isothermal amplification assays in GMO analysis";
RL   Food Control 84:278-283 (2018).
RX   DOI=10.1016/j.foodcont.2017.08.012
XX
RN   [2]
RP   1-199
RA   Wang C., Li R., Quan S., Shen P., Zhang D., Shi J., Yang L.;
RT   "GMO detection in food and feed through screening by visual loop-mediated isothermal amplification assays";
RL   Anal. and Bioanal. Chem.  407:4829-4834(2015).
RX   DOI=10.1007/s00216-015-8652-z
XX
RN   [3]
RP   1-199
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2017).
RX   PCR=QL-ELE-00-028.pdf
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..199
FT                   /standard_name="LAMP 199 bp amplicon"
FT                   /note="element-specific LAMP assay"
FT                   /target="phosphinothricin N-acetyltransferase (pat) gene from Streptomyces viridochromogenes"
FT   primer_bind     1..20
FT                   /standard_name="Primer forward: pat F3"
FT                   /note="GGCGCAAGGTTTTAAGTCTG"
FT                   /target="pat"
FT   primer_bind     join(32..51,complement(76..95))
FT                   /standard_name="Primer: pat FIP"
FT                   /note="TACCCCGGGCTGTGTATCCCATAGGCCTTCCAAACGATCC
FT                   "
FT   primer_bind     32..51
FT                   /note="ATAGGCCTTCCAAACGATCC"
FT                   /target="pat F2"
FT   primer_bind     complement(52..74)
FT                   /standard_name="Primer: pat lool F (complementary to the sequence between F1 and F2)"
FT                   /note="AAGCCTCATGCAACCTAACAGAT"
FT   primer_bind     complement(76..95)
FT                   /note="TACCCCGGGCTGTGTATCCC"
FT                   /target="pat F1c (complementary to F1)"
FT   primer_bind     join(97..117,complement(160..177))
FT                   /standard_name="Primer: pat BIP"
FT                   /note="ATTGCGCGCAGCTGGATACAAGGAGGAGCTGGCAACTCA"
FT   primer_bind     97..117
FT                   /note="ATTGCGCGCAGCTGGATACAA"
FT                   /target="pat B1c (complementary to B1)"
FT   primer_bind     118..156
FT                   /standard_name="Primer: pat lool B (complementary to the sequence between B1 and B2)"
FT                   /note="GCATGGTGGATGGCATGATGTTGGTTTTTGGCAAAGGGA"
FT   primer_bind     complement(160..177)
FT                   /note="GGAGGAGCTGGCAACTCA"
FT                   /target="pat B2"
FT   primer_bind     complement(181..199)
FT                   /standard_name="Primer reverse: pat B3"
FT                   /note="GGTAACTGGCCTAACTGGC"
FT                   /target="pat"
XX
SQ   Sequence 199 BP; 41 A; 41 C; 61 G; 56 T; 0 other;
     ggcgcaaggt tttaagtctg nnnnnnnnnn nataggcctt ccaaacgatc catctgttag        60
     gttgcatgag gcttngggat acacagcccg gggtanattg cgcgcagctg gatacaagca       120
     tggtggatgg catgatgttg gtttttggca aagggannnt gagttgccag ctcctccnnn       180
     gccagttagg ccagttacc                                                    199
//