ID   QL-ELE-00-026; SV 0; linear; genomic DNA; STS; SYN; 60 BP.
XX
AC   ;
XX
DT   04-AUG-2009
DT   23-DEC-2016
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DE   Qualitative duplex PCR method for detection of pat gene; bar gene (partim bar) (Debode et al., 2016).
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KW   element_specific.
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RN   [1]
RP   1-60
RA   Debode F., Huber I., Macarthur R., Rischitor P.E., Mazzara M., Herau V., Sebah D., Dobnik D., Broeders S., Roosens N.H., Busch U., Berben G., Morisset D., Zel J.;
RT   "Inter-laboratory studies for the validation of two singleplex (tE9 and pea lectin) and one duplex (pat/bar) real-time PCR methods for GMO detection";
RL   Food Control 73:452-461 (2016).
RX   DOI=10.1016/j.foodcont.2016.08.037.
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RN   [2]
RP   1-60
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2016).
RX   PCR=QL-ELE-00-026.pdf
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..60
FT                   /standard_name="PCR 60 bp amplicon"
FT                   /note="element-specific RT-PCR"
FT                   /target="phosphinothricin N-acetyltransferase (bar) gene from bacterium Streptomyces hygroscopicus"
FT   primer_bind     1..19
FT                   /standard_name="Primer forward: RapB-F1"
FT                   /note="ACAAGCACGGTCAACTTCC"
FT                   /target="bar"
FT   primer_bind     21..38
FT                   /standard_name="RT-PCR probe: RapB-S1"
FT                   /note="FAM-TACCGAGCCGCAGGAACC-BBQ"
FT   primer_bind     complement(43..60)
FT                   /standard_name="Primer reverse: RapB-R1"
FT                   /note="GAGGTCGTCCGTCCACTC"
FT                   /target="bar"
XX
SQ   Sequence 60 BP; 16 A; 20 C; 18 G; 6 T; 0 other;
     acaagcacgg tcaacttccn taccgagccg caggaaccnn nngagtggac ggacgacctc        60
//