ID   QL-ELE-00-021; SV 1; linear; other DNA; STD; UNA; 109 BP.
XX
AC   ;
XX
DT   25-JAN-2010
DT   29-FEB-2016
XX
DE   Qualitative PCR method for detection of phosphinothricin N-acetyltransferase (pat) gene (Barbau-Piednoir et al., 2014).
XX
KW   element_specific.
XX
RN   [1]
RP   1-109
RA   Barbau-Piednoir E., Stragier P., Roosens N., Mazzara M., Van den Eede G.,
RA   Van den Bulcke M.;
RT   "Inter-laboratory Testing of GMO Detection by Combinatory SYBR Green PCR
RT   Screening(CoSYPS)";
RL   Food Analitical Methods 0:0-0 (2014).
RX   DOI=10.1007/s12161-014-9837-3
XX
RN   [2]
RP   1-109
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2014).
RX   PCR=QL-ELE-00-021.pdf
XX
DR   GMOMETHODS; QL-TAX-BN-003;
DR   GMOMETHODS; QL-TAX-GM-001;
DR   GMOMETHODS; QL-TAX-ZM-002;
DR   GMOMETHODS; QL-PLN-00-006;
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    1..109
FT                   /note="Sybricon005 insert containing Streptomyces
FT                   viridochromogenes partial bar gene"
FT   STS             1..109
FT                   /standard_name="PCR 109 bp amplicon"
FT                   /note="element-specific PCR"
FT                   /target="phosphinothricin N-acetyltransferase (pat) gene from Streptomyces viridochromogenes"
FT   primer_bind     1..20
FT                   /standard_name="Primer forward: Pat-Pat Fwd"
FT                   /note="CCGCGGTTTGTGATATCGTT"
FT                   /target="pat"
FT   primer_bind     complement(85..109)
FT                   /standard_name="Primer reverse: Pat-Pat Rev"
FT                   /note="TCTTGCAACCTCTCTAGATCATCAA"
FT                   /target="pat"
XX
SQ   Sequence 109 BP; 35 A; 21 C; 27 G; 26 T; 0 other;
     ccgcggtttg tgatatcgtt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
     nnnnnnnnnn nnnnnnnnnn nnnnttgatg atctagagag gttgcaaga                   109
//