ID   QL-ELE-00-019; SV 1; linear; genomic DNA; STD; SYN; 108 BP.
XX
AC   ;
XX
DT   08-JAN-2003
DT   29-FEB-2016
XX
DE   Qualitative PCR method for detection of CP4 epsps gene (Barbau-Piednoir et al., 2014).
XX
KW   element_specific.
XX
RN   [1]
RP   1-108
RA   Barbau-Piednoir E., Stragier P., Roosens N., Mazzara M., Van den Eede G.,
RA   Van den Bulcke M.;
RT   "Inter-laboratory Testing of GMO Detection by Combinatory SYBR Green PCR
RT   Screening(CoSYPS)";
RL   Food Analitical Methods 0:0-0 (2014).
RX   DOI=10.1007/s12161-014-9837-3
XX
RN   [2]
RP   1-108
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2014).
RX   PCR=QL-ELE-00-019.pdf
XX
DR   GMOMETHODS; QL-TAX-BN-003;
DR   GMOMETHODS; QL-TAX-GM-001;
DR   GMOMETHODS; QL-TAX-ZM-002;
DR   GMOMETHODS; QL-PLN-00-006;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..108
FT                   /standard_name="PCR 108 bp amplicon"
FT                   /note="element-specific duplex PCR"
FT                   /target="CP4 epsps gene from Agrobacterium tumefasciens type I and II"
FT   primer_bind     1..18
FT                   /standard_name="Primer forward: CP4 Synthetic F"
FT                   /note="GCATGCTTCACGGTGCAA"
FT                   /target="CP4-EPSPS I"
FT   primer_bind     complement(90..108)
FT                   /standard_name="Primer reverse: CP4 Synthetic R"
FT                   /note="TGAAGGACCGGTGGGAGAT"
FT                   /target="CP4-EPSPS II"
FT   primer_bind     complement(90..108)
FT                   /standard_name="Primer reverse: CP4 synth Rbis"
FT                   /note="TGAAGGACCTGTGGGAGAT"
FT                   /target="CP4-EPSPS"
XX
SQ   Sequence 108 BP; 19 A; 45 C; 25 G; 19 T; 0 other;
     gcatgcttca cggtgcaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tctcccaccg gtccttca                    108
//