Perform your search by keyword, select a GMO unique identifier or click a link in the section below.

ID   QL-ELE-00-014; SV 0; linear; genomic DNA; STS; SYN; 60 BP.
AC   ;
DT   04-AUG-2009
DT   05-OCT-2010
DE   Qualitative PCR method for detection of phosphinothricin N-acetyltransferase (bar) gene
DE   (Grohmann et al., 2009).
KW   element_specific.
RN   [1]
RP   1-60
RA   Grohmann L., Brunen-Nieweler C., Nemeth A., Waiblinger H.U.;
RT   "Collaborative trial validation studies of real-time PCR-based GMO
RT   screening methods for detection of the bar gene and the ctp2-cp4epsps
RT   construct";
RL   J. Agric. Food Chem. 57:8913-8920 (2009).
RX   PUBMED; 19807158.
RX   DOI=10.1021/jf901598r
RN   [2]
RP   1-60
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QL-ELE-00-014.pdf
FH   Key             Location/Qualifiers
FT   STS             1..60
FT                   /standard_name="PCR 60 bp amplicon"
FT                   /note="element-specific RT-PCR"
FT                   /target="phosphinothricin N-acetyltransferase (bar) gene from bacterium Streptomyces hygroscopicus"
FT   primer_bind     1..19
FT                   /standard_name="Primer forward: RapB-F1"
FT                   /note="ACAAGCACGGTCAACTTCC"
FT                   /target="bar"
FT   primer_bind     20..42
FT                   /standard_name="RT-PCR probe: RapB-S1"
FT                   /note="FAM-TACCGAGCCGCAGGAACC-TAMRA"
FT   primer_bind     complement(43..60)
FT                   /standard_name="Primer reverse: RapB-R1"
FT                   /note="GAGGTCGTCCGTCCACTC"
FT                   /target="bar"
SQ   Sequence 60 BP; 10 A; 12 C; 10 G; 5 T; 23 other;
     acaagcacgg tcaacttccn nnnnnnnnnn nnnnnnnnnn nngagtggac ggacgacctc        60