ID   QL-ELE-00-014; SV 0; linear; genomic DNA; STS; SYN; 60 BP.
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AC   ;
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DT   04-AUG-2009
DT   05-OCT-2010
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DE   Qualitative PCR method for detection of phosphinothricin N-acetyltransferase gene
DE   (Grohmann et al., 2009).
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KW   element_specific.
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RN   [1]
RP   1-60
RA   Grohmann L., Brunen-Nieweler C., Nemeth A., Waiblinger H.U.;
RT   "Collaborative trial validation studies of real-time PCR-based GMO
RT   screening methods for detection of the bar gene and the ctp2-cp4epsps
RT   construct";
RL   J. Agric. Food Chem. 57:8913-8920 (2009).
RX   PUBMED; 19807158.
RX   DOI=10.1021/jf901598r
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RN   [2]
RP   1-60
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QL-ELE-00-014.pdf
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FH   Key             Location/Qualifiers
FH
FT   STS             1..60
FT                   /standard_name="PCR 60 bp amplicon"
FT                   /note="element-specific RT-PCR"
FT                   /target="phosphinothricin N-acetyltransferase (bar) gene from bacterium Streptomyces hygroscopicus"
FT   primer_bind     1..19
FT                   /standard_name="Primer forward: RapB-F1"
FT                   /note="ACAAGCACGGTCAACTTCC"
FT                   /target="bar"
FT   primer_bind     21..38
FT                   /standard_name="RT-PCR probe: RapB-S1"
FT                   /note="FAM-TACCGAGCCGCAGGAACC-TAMRA"
FT   primer_bind     complement(43..60)
FT                   /standard_name="Primer reverse: RapB-R1"
FT                   /note="GAGGTCGTCCGTCCACTC"
FT                   /target="bar"
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SQ   Sequence 60 BP; 16 A; 20 C; 18 G; 6 T; 0 other;
     acaagcacgg tcaacttccn taccgagccg caggaaccnn nngagtggac ggacgacctc        60
//