ID   QL-ELE-00-013; SV 0; linear; genomic DNA; STS; SYN; 84 BP.
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AC   ;
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DT   25-MAY-2009
DT   04-OCT-2010
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DE   Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim T-nos)
DE   (Waiblinger et al., 2008).
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KW   element_specific.
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RN   [1]
RP   1-84
RA   Waiblinger H.-U., Ernst B., Anderson A., Pietsch K.;
RT   "Validation and collaborative study of a P35S and T-nos duplex real-time
RT   PCR screening method to detect genetically modified organisms in food
RT   products";
RL   Eur. Food Res. Technol. 226:1221-1228 (2008).
RX   DOI=10.1007/s00217-007-0748-z
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RN   [2]
RP   1-84
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QL-ELE-00-013.pdf
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FH   Key             Location/Qualifiers
FH
FT   STS             1..84
FT                   /standard_name="PCR 84 bp amplicon"
FT                   /note="element-specific duplex RT-PCR"
FT                   /target="nopaline synthase terminator (T-nos) from Agrobacterium tumefaciens"
FT   primer_bind     1..25
FT                   /standard_name="Primer forward: 180-F"
FT                   /note="CATGTAATGCATGACGTTATTTATG"
FT                   /target="T-nos"
FT   primer_bind     28..55
FT                   /standard_name="Probe: TM-180YY"
FT                   /note="YY-ATGGGTTTTTATGATTAGAGTCCCGCAA-BHQ1"
FT   primer_bind     complement(60..84)
FT                   /standard_name="Primer reverse: 180-R"
FT                   /note="TTGTTTTCTATCGCGTATTAAATGT"
FT                   /target="T-nos"
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SQ   Sequence 84 BP; 29 A; 11 C; 16 G; 28 T; 0 other;
     catgtaatgc atgacgttat ttatgnnatg ggtttttatg attagagtcc cgcaannnna        60
     catttaatac gcgatagaaa acaa                                               84
//