ID   QL-ELE-00-011; SV 0; linear; genomic DNA; STS; SYN; 84 BP.
XX
AC   ;
XX
DT   25-MAY-2009
DT   13-JUN-2017
XX
DE   Qualitative PCR method for detection of nopaline synthase terminator (T-nos).
XX
KW   element_specific.
XX
RN   [1]
RP   1-84
RA   Reiting R., Broll H., Waiblinger H.-U., Grohmann L.;
RT   "Collaborative Study of a T-nos Real-Time PCR Method for Screening of
RT   Genetically Modified Organisms in Food Products";
RL   J. Verbr. Lebensm. 2:116-121 (2007).
RX   DOI=10.1007/s00003-007-0189-4
XX
RN   [2]
RP   1-84
RT   "Foodstuffs--Methods of analysis for the detection of genetically modified organisms and derived products -- Qualitative nucleic acid based methods Amendment 1";
RL   ISO/TS 21569:2005/Amd 1:2013 (2013).
RX   ISO=56164
XX
RN   [3]
RP   1-84
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QL-ELE-00-011.pdf
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..84
FT                   /standard_name="PCR 84 bp amplicon"
FT                   /note="element-specific RT-PCR"
FT                   /target="Nopaline synthase terminator (T-nos)"
FT   primer_bind     1..25
FT                   /standard_name="Primer forward: 180-F"
FT                   /note="CATGTAATGCATGACGTTATTTATG"
FT                   /target="T-nos"
FT   primer_bind     28..55
FT                   /standard_name="RT-PCR probe: Tm-180"
FT                   /note="FAM-ATGGGTTTTTATGATTAGAGTCCCGCAA-TAMRA"
FT   primer_bind     complement(60..84)
FT                   /standard_name="Primer reverse: 180-R"
FT                   /note="TTGTTTTCTATCGCGTATTAAATGT"
FT                   /target="T-nos"
XX
SQ   Sequence 84 BP; 29 A; 11 C; 16 G; 28 T; 0 other;
     catgtaatgc atgacgttat ttatgnnatg ggtttttatg attagagtcc cgcaannnna        60
     catttaatac gcgatagaaa acaa                                               84
//