ID   QT-TAX-ZM-008; SV 0; linear; genomic DNA; STS; PLN; 79 BP.
XX
AC   ;
XX
DT   29-JUL-2023
DT   12-JUN-2024
XX
DE   Quantitative PCR method for detection of maize high-mobility-group gene
XX
KW   taxon_specific, validated_in_combination.
XX
OS   Zea mays (maize)
XX
RN   [1]
RP   1-79
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Maize DP202216 Using
RT   Real-time PCR - Validation Report";
RL   Online Publication (2023).
RX   EURL_GMFF=EURL-VL-03-19-VR.pdf
RX   EURL_GMFF=EURL-VL-03-19-VM.pdf
XX
RN   [2]
RP   1-79
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Maize DP23211 Using
RT   Real-time PCR - Validation Report";
RL   Online Publication (2023).
RX   EURL_GMFF=EURL-VL-09-19-VR.pdf
RX   EURL_GMFF=EURL-VL-09-19-VM.pdf
XX
RN   [3]
RP   1-79
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Maize DP910521 Using Real-time PCR - Validation Report";
RL   Online Publication (2024).
RX   EURL_GMFF=EURL-VL-04-21-VR.pdf
RX   EURL_GMFF=EURL-VL-04-21-VM.pdf
XX
RN   [4]
RP   1-79
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Maize event DP51291 Using Real-time PCR - Validation Report"; 
RL   Online Publication (2024).
RX   EURL_GMFF=EURL-VL-04-22-VR.pdf
RX   EURL_GMFF=EURL-VL-04-22-VM.pdf
XX
RN   [5]
RP   1-79
RT   "See Cross-references below";
RL   Online Publication (2023).
XX
DR   GMOMETHODS; QT-EVE-ZM-034;
DR   GMOMETHODS; QT-EVE-ZM-035;
DR   GMOMETHODS; QT-EVE-ZM-038;
DR   GMOMETHODS; QT-EVE-ZM-039;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..79
FT                   /standard_name="PCR 79 bp amplicon"
FT                   /note="taxon-specific RT-PCR for maize"
FT                   /target="high-mobility-group (hmg) gene"
FT   primer_bind     1..23
FT                   /standard_name="Primer forward: ZM1-F"
FT                   /note="TTGGACTAGAAATCTCGTGCTGA"
FT                   /target="hmg"
FT   primer_bind     complement(34..56)
FT                   /standard_name="RT-PCR probe: Probe ZM1"
FT                   /note="FAM-CAATCCACACAAACGCACGCGTA-BHQ1"
FT   primer_bind     complement(58..79)
FT                   /standard_name="Primer reverse: ZM1-R"
FT                   /note="GCTACATAGGGAGCCTTGTCCT"
FT                   /target="hmg"
XX
SQ   Sequence 79 BP; 16 A; 13 C; 22 G; 28 T; 0 other;
     ttggactaga aatctcgtgc tgannnnnnn nnntacgcgt gcgtttgtgt ggattgnagg        60
     acaaggctcc ctatgtagc                                                     79
//