ID   QT-TAX-ZM-006; SV 0; linear; genomic DNA; STS; PLN; 151 BP.
XX
AC   ;
XX
DT   15-SEP-2009
DT   16-SEP-2010
XX
DE   Quantitative PCR method for detection of maize starch synthase IIb gene
XX
KW   taxon_specific, validated_in_combination.
XX
OS   Zea mays (maize)
XX
RN   [1]
RP   1-151
RA   Shindo Y., Kuribara H., Matsuoka T., Futo S., Sawada C., Shono J.,
RA   Akiyama H., Goda Y., Toyoda M., Hino A.;
RT   "Validation of real-time PCR analyses for line-specific quantitation of
RT   genetically modified maize and soybean using new reference molecules";
RL   J AOAC Int 85:1119-1126 (2002).
RX   PUBMED; 12374412.
XX
RN   [2]
RP   1-151
RT   "Foodstuffs - Methods of analysis for the detection of genetically
RT   modified organisms and derived products - Quantitative nucleic acid based
RT   methods";
RL   ISO 21570:1-103 (2005).
RX   ISO=34615
XX
RN   [3]
RP   1-151
RT   "See Cross-references below";
RL   Online Publication (2010).
XX
DR   GMOMETHODS; QT-CON-00-006;
DR   GMOMETHODS; QT-CON-00-007;
DR   GMOMETHODS; QT-CON-00-004;
DR   GMOMETHODS; QT-CON-00-005;
DR   GMOMETHODS; QT-CON-00-008;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..151
FT                   /standard_name="PCR 151 bp amplicon"
FT                   /note="Taxon-specific RT-PCR for maize"
FT                   /target="maize starch synthase IIb (zSSIIb) gene"
FT   primer_bind     1..22
FT                   /standard_name="Primer forward: SSllb 1-5'"
FT                   /note="CTCCCAATCCTTTGACATCTGC"
FT                   /target="zSSIIb"
FT   primer_bind     28..52
FT                   /standard_name="RT-PCR probe: SSIIb-Taq"
FT                   /note="FAM-AGCAAAGTCAGAGCGCTGCAATGCA-TAMRA"
FT   primer_bind     complement(129..151)
FT                   /standard_name="Primer reverse: SSIIb 1-3'"
FT                   /note="TCGATTTCTCTCTTGGTGACAGG"
FT                   /target="zSSIIb"
XX
SQ   Sequence 151 BP; 41 A; 48 C; 40 G; 22 T; 0 other;
     ctcccaatcc tttgacatct gcnnnnnagc aaagtcagag cgctgcaatg cannnnnnnn        60
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       120
     nnnnnnnncc tgtcaccaag agagaaatcg a                                      151
//