ID   QT-TAX-SL-002; SV 0; linear; genomic DNA; STS; PLN; 92 BP.
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AC   ;
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DT   23-JUN-2009
DT   20-SEP-2010
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DE   Quantitative PCR method for detection of tomato LAT52
DE   (Yang et al., 2008).
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KW   taxon_specific, validated_independently.
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OS   Solanum lycopersicum (tomato)
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RN   [1]
RP   1-92
RA   Yang L., Zhang H., Guo J., Pan L., Zhang D.;
RT   "International Collaborative Study of the Endogenous Reference Gene LAT52
RT   Used for Qualitative and Quantitative Analyses of Genetically Modified
RT   Tomato";
RL   J. Agric. Food Chem. 56:3438-3443 (2008).
RX   DOI=10.1021/jf073464q
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RN   [2]
RP   1-92
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QT-TAX-SL-002.pdf
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FH   Key             Location/Qualifiers
FH
FT   STS             1..92
FT                   /standard_name="PCR 92 bp amplicon"
FT                   /note="Taxon-specific RT-PCR for tomato"
FT                   /target="LAT52 gene"
FT   primer_bind     1..22
FT                   /standard_name="Primer forward: Lat1"
FT                   /note="AGACCACGAGAACGATATTTGC"
FT                   /target="LAT52"
FT   primer_bind     complement(41..64)
FT                   /standard_name="RT-PCR probe: Lp"
FT                   /note="HEX-CTCTTTGCAGTCCTCCCTTGGGCT-BHQ"
FT   primer_bind     complement(69..92)
FT                   /standard_name="Primer reverse: Lat2"
FT                   /note="TTCTTGCCTTTTCATATCCAGACA"
FT                   /target="LAT52"
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SQ   Sequence 92 BP; 34 A; 13 C; 30 G; 15 T; 0 other;
     agaccacgag aacgatattt gcnnnnnnnn nnnnnnnnnn agcccaaggg aggactgcaa        60
     agagnnnntg tctggatatg aaaaggcaag aa                                      92
//