ID   QT-TAX-ST-010; SV 0; linear; genomic DNA; STS; PLN; 89 BP.
XX
AC   ;
XX
DT   27-JUN-2008
DT   28-OCT-2010
XX
DE   Quantitative PCR method for detection of potato UDP-glucose pyrophosphorylase gene
DE   (Savini et al., 2006).
XX
KW   taxon_specific, validated_in_combination.
XX
OS   Solanum tuberosum (potato)
XX
RN   [1]
RP   1-89
RA   Savini C., Foti N., Mazzara M., Charles Delobel C., Van Den Eede G.;
RT   "Event-specific Method for the Quantification of Event EH92-527-1 Potato Using Real-time PCR - Validation Report and Protocol - Sampling and DNA Extraction of Potato";
RL   Online Publication (2006).
RX   DOI=10.2788/30418
XX
RN   [2]
RP   1-89
RT   "See Cross-references below";
RL   Online Publication (2010).
XX
DR   GMOMETHODS; QT-EVE-ST-001;
XX
FH   Key             Location/Qualifiers
FH
FT   gene            1..89
FT                   /gene="UGPase"
FT                   /allele="Lemhi10"
FT   STS             complement(1..89)
FT                   /standard_name="PCR 88 bp amplicon"
FT                   /note="taxon-specific RT-PCR for potato"
FT                   /target="UDP-glucose pyrophosphorylase (UGPase) gene"
FT   primer_bind     1..21
FT                   /standard_name="Primer forward: UGP-af7"
FT                   /note="GGACATGTGAAGAGACGGAGC"
FT                   /target="UGPase"
FT   primer_bind     complement(36..62)
FT                   /standard_name="RT-PCR probe: UGP-sf1"
FT                   /note="FAM-CTACCACCATTACCTCGCACCTCCTCA-TAMRA"
FT   primer_bind     complement(70..89)
FT                   /standard_name="Primer reverse: UGP-ar8"
FT                   /note="CCTACCTCTACCCCTCCGC"
FT                   /note="Based on the sequence coming
FT                   from GenBank record with accession number U20345, between
FT                   the 17th and 18th nucleotide of the reverse primer (C and G, respectively) is
FT                   expected an additional nucleotide A. In the CRL dossier
FT                   concerning GMO event EH92-527-1 potato this 2250th
FT                   nucleotide of the GenBank sequence is deleted"
FT                   /target="UGPase"
XX
SQ   Sequence 89 BP; 18 A; 5 C; 35 G; 10 T; 21 other;
     ggacatgtga agagacggag cnnnnnnnnn nnnnntgagg aggtgcgagg taatggtggt        60
     agnnnnnnng cnggaggggt agaggtagg                                          89
//