ID   QL-TAX-ZM-002; SV 1; linear; genomic DNA; STD; PLN; 84 BP.
XX
AC   ;
XX
DT   03-JUN-1988
DT   29-FEB-2016
XX
DE   Qualitative PCR method for detection of maize alcoholdeydrogenase 1 gene (Barbau-Piednoir et al., 2014).
XX
KW   taxon_specific, validated_in_combination.
XX
OS   Zea mays (maize)
XX
RN   [1]
RP   1-84
RA   Barbau-Piednoir E., Stragier P., Roosens N., Mazzara M., Van den Eede G.,
RA   Van den Bulcke M.;
RT   "Inter-laboratory Testing of GMO Detection by Combinatory SYBR Green PCR
RT   Screening(CoSYPS)";
RL   Food Analitical Methods 0:0-0 (2014).
RX   DOI=10.1007/s12161-014-9837-3
XX
RN   [2]
RP   1-84
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2014).
RX   PCR=QL-TAX-ZM-002.pdf
XX
RN   [3]
RP   1-84
RT   "See Cross-references below";
RL   Online Publication (2014).
XX
DR   GMOMETHODS; QL-ELE-00-017;
DR   GMOMETHODS; QL-ELE-00-018;
DR   GMOMETHODS; QL-ELE-00-019;
DR   GMOMETHODS; QL-ELE-00-020;
DR   GMOMETHODS; QL-ELE-00-021;
DR   GMOMETHODS; QL-ELE-00-022;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..84
FT                   /standard_name="PCR 84 bp amplicon"
FT                   /note="taxon-specific PCR for maize"
FT                   /target="alcohol dehydrogenase 1 (adh1) gene"
FT   primer_bind     1..27
FT                   /standard_name="Primer forward: ADH_alt Fwd"
FT                   /note="TCTCTTCCTCCTTTAGAGCTACCACTA"
FT                   /target="adh1"
FT   primer_bind     complement(64..84)
FT                   /standard_name="Primer reverse: ADH_alt Rev"
FT                   /note="AATCGATCCAAAGCGAGATGA"
FT                   /target="adh1"
XX
SQ   Sequence 84 BP; 16 A; 26 C; 12 G; 30 T; 0 other;
     tctcttcctc ctttagagct accactannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
     nnntcatctc gctttggatc gatt                                               84
//