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ID   QT-TAX-OS-003; SV 0; linear; genomic DNA; STS; PLN; 81 BP.
AC   ;
DT   23-JUN-2009
DT   26-AGO-2011
DE   Quantitative PCR method for detection of rice sucrose-phosphate synthase gene
DE   (Jiang et al., 2009).
KW   taxon_specific, validated_independently.
OS   Oryza sativa (rice)
RN   [1]
RP   1-81
RA   Jiang L., Yang L., Zhang H., Guo J., Mazzara M., Van den Eede G., Zhang
RA   D.;
RT   "International collaborative study of the endogenous reference gene,
RT   sucrose phosphate synthase (SPS), used for qualitative and quantitative
RT   analysis of genetically modified rice";
RL   J. Agric. Food Chem. 57:3525-3532 (2009).
RX   PUBMED; 19326953.
RX   DOI=10.1021/jf803166p
RN   [2]
RP   1-81
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QT-TAX-OS-003.pdf
FH   Key             Location/Qualifiers
FT   STS             1..81
FT                   /standard_name="PCR 81 bp amplicon"
FT                   /note="Taxon-specific RT-PCR for rice"
FT                   /target="sucrose-phosphate synthase (SPS) gene"
FT   primer_bind     1..18
FT                   /standard_name="Primer forward: SPS-1F"
FT                   /note="TTGCGCCTGAACGGATAT"
FT                   /target="SPS"
FT   primer_bind     complement(42..60)
FT                   /standard_name="RT-PCR probe: SPS-P"
FT                   /note="HEX-TCCGAGCCGTCCGTGCGTC-TAMRA"
FT   primer_bind     complement(62..81)
FT                   /standard_name="Primer reverse: SPS-3R"
FT                   /note="CGGTTGATCTTTTCGGGATG"
FT                   /target="SPS"
SQ   Sequence 81 BP; 21 A; 23 C; 20 G; 17 T; 0 other;
     ttgcgcctga acggatatnn nnnnnnnnnn nnnnnnnnnn ngacgcacgg acggctcgga        60
     ncatcccgaa aagatcaacc g                                                  81