ID   QT-EVE-BN-014; SV 0; linear; genomic DNA; STD; PLN; 89 BP.
XX
AC   MON-94100-2;
XX
DT   30-AUG-2022
DT   30-AUG-2022
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DE   Quantitative PCR method for detection of oilseed rape event MON94100 (EURL GMFF, 2022).
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KW   event_specific.
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OS   Brassica napus (rape) - event MON94100 (MON-94100-2)
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RN   [1]
RP   1-89
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Oilseed Rape MON 94100
RT   Using Real-time PCR - Validation Report";
RL   Online Publication (2022).
RX   EURL_GMFF=EURL-VL-04-20-VR.pdf
RX   EURL_GMFF=EURL-VL-04-20-VM.pdf
XX
RN   [2]
RP   1-89
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2022).
RX   PCR=QT-EVE-BN-014.pdf
XX
DR   GMOMETHODS; QT-TAX-BN-001;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..89
FT                   /note="Event-specific RT-PCR"
FT                   /target="5' integration border region (IBR) between the insert of oilseed rape event MON94100 and the oilseed rape host genome"
FT   primer_bind     1..31
FT                   /standard_name="Primer forward: MON 94100 primer 1"
FT                   /note="CACCATCTAATGAATAGTCACCAAAATAACG"
FT                   /target="5'-host genome"
FT   primer_bind     complement(45..63)
FT                   /standard_name="RT-PCR probe: MON 94100 probe"
FT                   /note="FAM-TGATGCTGACTGGTGTCAA-MGBNFQ"
FT   primer_bind     complement(65..89)
FT                   /standard_name="Primer reverse: MON 94100 primer 2"
FT                   /note="CTATTCGGGCCTAACTTTTGGTGTG"
FT                   /target="insert"
XX
SQ   Sequence 89 BP; 33 A; 24 C; 13 G; 19 T; 0 other;
     caccatctaa tgaatagtca ccaaaataac gnnnnnnnnn nnnnttgaca ccagtcagca        60
     tcancacacc aaaagttagg cccgaatag                                          89
//