Perform your search by keyword, select a GMO unique identifier or click a link in the section below.

ID   QL-ELE-00-026; SV 0; linear; genomic DNA; STS; SYN; 60 BP.
AC   ;
DT   04-AUG-2009
DT   23-DEC-2016
DE   Qualitative duplex PCR method for detection of pat gene; bar gene (partim bar) (Debode et al., 2016).
KW   element_specific.
RN   [1]
RP   1-60
RA   Debode F., Huber I., Macarthur R., Rischitor P.E., Mazzara M., Herau V., Sebah D., Dobnik D., Broeders S., Roosens N.H., Busch U., Berben G., Morisset D., Zel J.;
RT   "Inter-laboratory studies for the validation of two singleplex (tE9 and pea lectin) and one duplex (pat/bar) real-time PCR methods for GMO detection";
RL   Food Control 73:452-461 (2016).
RX   DOI=10.1016/j.foodcont.2016.08.037.
RN   [2]
RP   1-60
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2016).
RX   PCR=QL-ELE-00-026.pdf
FH   Key             Location/Qualifiers
FT   STS             1..60
FT                   /standard_name="PCR 60 bp amplicon"
FT                   /note="element-specific RT-PCR"
FT                   /target="phosphinothricin N-acetyltransferase (bar) gene from bacterium Streptomyces hygroscopicus"
FT   primer_bind     1..19
FT                   /standard_name="Primer forward: RapB-F1"
FT                   /note="ACAAGCACGGTCAACTTCC"
FT                   /target="bar"
FT   primer_bind     21..38
FT                   /standard_name="RT-PCR probe: RapB-S1"
FT                   /note="FAM-TACCGAGCCGCAGGAACC-BBQ"
FT   primer_bind     complement(43..60)
FT                   /standard_name="Primer reverse: RapB-R1"
FT                   /note="GAGGTCGTCCGTCCACTC"
FT                   /target="bar"
SQ   Sequence 60 BP; 10 A; 12 C; 10 G; 5 T; 23 other;
     acaagcacgg tcaacttccg taccgagccg caggaaccgc aggagtggac ggacgacctc        60