ID   QT-EVE-GM-017; SV 0; linear; genomic DNA; STS; SYN; 88 BP.
XX
AC   SYN-000H2-5;
XX
DT   24-AUG-2012
DT   24-AUG-2016
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DE   Quantitative PCR method for detection of soybean event SYHT0H2 (EURL GMFF, 2016).
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KW   event_specific.
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OS   Glycine max (soybean) - event SYHT0H2 (SYN-000H2-5)
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RN   [1]
RP   1-88
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Soybean SYHT0H2 by Real-time PCR - JRC Validated Methods, Reference Methods and Measurements Reports";
RL   Online Publication (2016).
RX   EURL_GMFF=EURL-VL-04-12-VR.pdf
RX   EURL_GMFF=EURL-VL-04-12-VP.pdf
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RN   [2]
RP   1-88
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2016).
RX   PCR=QT-EVE-GM-017.pdf
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DR   GMOMETHODS; QT-TAX-GM-002;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..88
FT                   /standard_name="PCR 88 bp amplicon"
FT                   /note="Event-specific RT-PCR;
FT                   /target="3' integration border region (IBR) between the insert of soybean event SYHT0H2 and the soybean host genome"
FT   primer_bind     1..19
FT                   /standard_name="Primer forward: FE08316-F"
FT                   /note="GGGAATTGGGTACCATGCC"
FT                   /target="insert"
FT   primer_bind     27..47
FT                   /standard_name="RT-PCR probe: FE08318-P"
FT                   /note="FAM-CCAGCATGGCCGTATCCGCAA-BHQ"
FT   primer_bind     complement(68..88)
FT                   /standard_name="Primer reverse: FE08317-R"
FT                   /note="TGTGTGCCATTGGTTTAGGGT"
FT                   /target="3'-host genome"
XX
SQ   Sequence 88 BP; 23 A; 23 C; 23 G; 19 T; 0 other;
     gggaattggg taccatgccn nnnnnnccag catggccgta tccgcaannn nnnnnnnnnn        60
     nnnnnnnacc ctaaaccaat ggcacaca                                           88
//