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ID   QT-EVE-BN-012; SV 0; linear; genomic DNA; STD; SYN; 128 BP.
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AC   BPS-BFLFK-2;
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DT   08-SEP-2021
DT   05-OCT-2022
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DE   Quantitative PCR method for detection of oilseed rape event LBFLFK locus1 (EURL GMFF, 2022).
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KW   event_specific.
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OS   Brassica napus (rape) - event LBFLFK (BPS-BFLFK-2)
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RN   [1]
RP   1-128
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Oilseed Rape LBFLFK
RT   Locus1 and Locus2 Using Real-time PCR - Validation Report";
RL   Online Publication (2022).
RX   EURL_GMFF=EURL-VL-02-19-VR.pdf
RX   EURL_GMFF=EURL-VL-02-19-VM.pdf
XX
RN   [2]
RP   1-128
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2022).
RX   PCR=QT-EVE-BN-012.pdf
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DR   GMOMETHODS; QT-TAX-BN-001;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..128
FT                   /note="Event-specific RT-PCR"
FT                   /target="3' integration border region (IBR) between the
FT                   insert of oilseed rape event LBFLFK locus1 and the
FT                   oilseed rape host genome"
FT   primer_bind     1..23
FT                   /standard_name="Primer forward: leftFST_ev1_loc1-f4"
FT                   /note="ACCATCATACTCATTGCTGATCC"
FT                   /target="insert"
FT   primer_bind     24..49
FT                   /standard_name="RT-PCR probe: leftFST_ev1_loc1-s2"
FT                   /note="FAM-ATGTAGATTTCCCGGACATGAAGCCA-BHQ1"
FT   primer_bind     complement(104..128)
FT                   /standard_name="Primer reverse: leftFST_ev1_loc1-r5"
FT                   /note="TTCCTGTATACGCACACATAGTATA"
FT                   /target="3'-host genome"
XX
SQ   Sequence 128 BP; 40 A; 27 C; 20 G; 41 T; 0 other;
     accatcatac tcattgctga tccatgtaga tttcccggac atgaagccan nnnnnnnnnn        60
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntatacta tgtgtgcgta       120
     tacaggaa                                                                128
//