ID QT-EVE-BN-012; SV 0; linear; genomic DNA; STD; SYN; 128 BP. XX AC BPS-BFLFK-2; XX DT 08-SEP-2021 DT 05-OCT-2022 XX DE Quantitative PCR method for detection of oilseed rape event LBFLFK locus1 (EURL GMFF, 2022). XX KW event_specific. XX OS Brassica napus (rape) - event LBFLFK (BPS-BFLFK-2) XX RN [1] RP 1-128 RA European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission; RT "Event-specific Method for the Quantification of Oilseed Rape LBFLFK RT Locus1 and Locus2 Using Real-time PCR - Validation Report"; RL Online Publication (2022). RX EURL_GMFF=EURL-VL-02-19-VR.pdf RX EURL_GMFF=EURL-VL-02-19-VM.pdf XX RN [2] RP 1-128 RT "PCR reactions set up and amplification conditions"; RL Online Publication (2022). RX PCR=QT-EVE-BN-012.pdf XX DR GMOMETHODS; QT-TAX-BN-001; XX FH Key Location/Qualifiers FH FT STS 1..128 FT /note="Event-specific RT-PCR" FT /target="3' integration border region (IBR) between the FT insert of oilseed rape event LBFLFK locus1 and the FT oilseed rape host genome" FT primer_bind 1..23 FT /standard_name="Primer forward: leftFST_ev1_loc1-f4" FT /note="ACCATCATACTCATTGCTGATCC" FT /target="insert" FT primer_bind 24..49 FT /standard_name="RT-PCR probe: leftFST_ev1_loc1-s2" FT /note="FAM-ATGTAGATTTCCCGGACATGAAGCCA-BHQ1" FT primer_bind complement(104..128) FT /standard_name="Primer reverse: leftFST_ev1_loc1-r5" FT /note="TTCCTGTATACGCACACATAGTATA" FT /target="3'-host genome" XX SQ Sequence 128 BP; 40 A; 27 C; 20 G; 41 T; 0 other; accatcatac tcattgctga tccatgtaga tttcccggac atgaagccan nnnnnnnnnn 60 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntatacta tgtgtgcgta 120 tacaggaa 128 //