ID   QT-TAX-GM-009; SV 0; linear; genomic DNA; STD; PLN; 105 BP.
XX
AC   ;
XX
DT   06-JUN-2008
DT   28-OCT-2010
XX
DE   Quantitative PCR method for detection of soybean lectin gene
DE   (Mazzara et al., 2007).
XX
KW   taxon_specific, validated_in_combination.
XX
OS   Glycine max (soybean)
XX
RN   [1]
RP   1-105
RA   Mazzara M., Charles Delobel C., Grazioli E., Larcher S., Savini C., Van Den Eede G.;
RT   "Event-Specific Method for the Quantification of Soybean Line A2704-12 Using Real-Time PCR- Validation Report and Protocol - Soybean Seeds Sampling and DNA Extraction";
RL   Online Publication (2007).
RX   DOI=10.2788/28149
XX
RN   [2]
RP   1-105
RT   "See Cross-references below";
RL   Online Publication (2010).
XX
DR   GMOMETHODS; QT-EVE-GM-004;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..105
FT                   /standard_name="PCR 105 bp amplicon"
FT                   /note="taxon-specific RT-PCR for soybean"
FT                   /target="lectin (Le1) gene"
FT   primer_bind     1..23
FT                   /standard_name="Primer forward: KVM164"
FT                   /note="CACCTTTCTCGCACCAATTGACA"
FT                   /target="Le1"
FT   misc_difference 2..3
FT                   /note="Two mismatches found: 1338th and 1339th nucleotide, based on the sequence coming from EMBL record with accession number K00821, is T and T, respectively. 2nd and 3rd nucleotide, based on CRL dossier concerning GMO event A2704-12 Soybean, is A and C, respectively."
FT   primer_bind     29..54
FT                   /standard_name="RT-PCR probe: TM021"
FT                   /note="FAM-CCACAAACACATGCAGGTTATCTTGG-TAMRA"
FT   primer_bind     complement(86..105)
FT                   /standard_name="Primer reverse: KVM165"
FT                   /note="TCAAACTCAACAGCGACGAC"
FT                   /target="Le1"
XX
SQ   Sequence 105 BP; 15 A; 18 C; 14 G; 22 T; 36 other;
     cttctttctc gcaccaattg acannnnncc acaaacacat gcaggttatc ttggnnnnnn        60
     nnnnnnnnnn nnnnnnnnnn nnnnngtcgt cgctgttgag tttga                       105
//