ID   QT-TAX-GM-007; SV 0; linear; genomic DNA; STS; PLN; 118 BP.
XX
AC   ;
XX
DT   15-SEP-2009
DT   21-SEP-2010
XX
DE   Quantitative PCR method for detection of soybean lectin gene
XX
KW   taxon_specific, validated_in_combination.
XX
OS   Glycine max (soybean)
XX
RN   [1]
RP   1-118
RA   Shindo Y., Kuribara H., Matsuoka T., Futo S., Sawada C., Shono J.,
RA   Akiyama H., Goda Y., Toyoda M., Hino A.;
RT   "Validation of real-time PCR analyses for line-specific quantitation of
RT   genetically modified maize and soybean using new reference molecules";
RL   J AOAC Int 85:1119-1126 (2002).
RX   PUBMED; 12374412.
XX
RN   [2]
RP   1-118
RT   "Foodstuffs - Methods of analysis for the detection of genetically
RT   modified organisms and derived products - Quantitative nucleic acid based
RT   methods";
RL   ISO 21570:1-103 (2005).
RX   ISO=34615
XX
RN   [3]
RP   1-118
RT   "See Cross-references below";
RL   Online Publication (2010).
XX
DR   GMOMETHODS; QT-CON-00-001;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..118
FT                   /standard_name="PCR 118 bp amplicon"
FT                   /note="Taxon-specific RT-PCR for soybean"
FT                   /target="lectin (Le1) gene"
FT   primer_bind     1..19
FT                   /standard_name="Primer forward: Le1n02-5'"
FT                   /note="GCCCTCTACTCCACCCCCA"
FT                   /target="Le1"
FT   primer_bind     55..80
FT                   /standard_name="RT-PCR probe: Le1-Taq"
FT                   /note="FAM-AGCTTCGCCGCTTCCTTCAACTTCAC-TAMRA"
FT   primer_bind     complement(99..118)
FT                   /standard_name="Primer reverse: Le1n02-3'"
FT                   /note="GCCCATCTGCAAGCCTTTTT"
FT                   /target="Le1"
XX
SQ   Sequence 118 BP; 27 A; 43 C; 22 G; 26 T; 0 other;
     gccctctact ccacccccan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagcttc        60
     gccgcttcct tcaacttcac nnnnnnnnnn nnnnnnnnaa aaaggcttgc agatgggc         118
//