ID   QL-EVE-EC-002; SV 0; linear; genomic DNA; STS; BCT; 85 BP.
XX
AC   ;
XX
DT   02-SEP-2008
DT   23-NOV-2017
XX
DE   Qualitative PCR method for detection of  E. coli K-12 event 19E (EURL GMFF, 2015).
XX
KW   event_specific.
XX
OS   Escherichia coli K-12 (bacteria) - event 19E
XX
RN   [1]
RP   1-85
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific method for the detection of dried-killed bacterial biomass PL73 (LM) derived from Escherichia coli K-12 GM strain 19E using real-time PCR - JRC Validated Methods, Reference Methods and Measurements Reports";
RL   Online Publication (2015).
RX   EURL_GMFF=CRL-VL-06-08-VR.pdf
RX   EURL_GMFF=CRL-VL-06-08-VP.pdf
XX
RN   [2]
RP   1-85
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2017).
RX   PCR=QL-EVE-EC-002.pdf
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..85
FT                   /standard_name="PCR 85 bp amplicon"
FT                   /note="Event-specific RT-PCR";
FT                   /target="5' integration border region (IBR) between the insert of E. coli K-12 event 19E and the bacterial host genome";                 
FT   primer_bind     1..27
FT                   /standard_name="Primer forward: LMA for"
FT                   /note="GGTTATCCAGTAATAGCCATCTTCATC"
FT                   /target="5'-host genome"
FT   primer_bind     38..63
FT                   /standard_name="RT-PCR probe: LMA probe"
FT                   /note="FAM-CCGTCGCCGCTGTATTGATTCACTTG-TAMRA"
FT   primer_bind     complement(64..85)
FT                   /standard_name="Primer reverse: LMA rev"
FT                   /note="CCTCCCGGTTTTTTTCGTACTT"
FT                   /target="insert"
XX
SQ   Sequence 85 BP; 22 A; 22 C; 22 G; 19 T; 0 other;
     ggttatccag taatagccat cttcatcnnn nnnnnnnccg tcgccgctgt attgattcac        60
     ttgaagtacg aaaaaaaccg ggagg                                              85
//