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ID   QT-TAX-ZM-003; SV 0; linear; genomic DNA; STS; PLN; 135 BP.
XX
AC   ;
XX
DT   29-MAY-2009
DT   09-MAY-2015
XX
DE   Quantitative PCR method for detection of maize alcoholdeydrogenase 1 gene (Mazzara et al., 2013).
XX
KW   taxon_specific,  validated_in_combination.
XX
OS   Zea mays (maize)
XX
RN   [1]
RP   1-135
RA   Mazzara M., Grazioli E., Savini C., Van Den Eede G.;
RT   "Event-specific Method for the Quantification of Maize Line T25 Using Real-time PCR v. 1.01 - Validation Report and Validated Method";
RL   Online Publication (2013).
RX   DOI=10.2788/18420
XX
RN   [2]
RP   1-135
RT   "See Cross-references below";
RL   Online Publication (2013).
XX
DR   GMOMETHODS; QT-EVE-ZM-011;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..135
FT                   /standard_name="PCR 135 bp amplicon"
FT                   /note="taxon-specific RT-PCR for maize"
FT                   /target="alcohol deydrogenase 1 (adh1) gene"
FT   primer_bind     1..24
FT                   /standard_name="Primer forward: ADH-FF3"
FT                   /note="CGTCGTTTCCCATCTCTTCCTCCT"
FT                   /target="adh1"
FT   primer_bind     44..70
FT                   /standard_name="RT-PCR probe: ADH1-MDO"
FT                   /note="FAM-AATCAGGGCTCATTTTCTCGCTCCTCA-TAMRA"
FT   primer_bind     complement(116..135)
FT                   /standard_name="Primer reverse: ADH-RR4"
FT                   /note="CCACTCCGAGACCCTCAGTC"
FT                   /target="adh1"
XX
SQ   Sequence 135 BP; 23 A; 36 C; 32 G; 44 T; 0 other;
     cgtcgtttcc catctcttcc tcctnnnnnn nnnnnnnnnn nnnaatcagg gctcattttc        60
     tcgctcctca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngactg       120
     agggtctcgg agtgg                                                        135
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