ID QT-EVE-GM-015; SV 0; linear; genomic DNA; STS; SYN; 99 BP. XX AC DAS-44406-6; XX DT 18-JUN-2012 DT 07-MAY-2015 XX DE Quantitative PCR method for detection of soybean event DAS-44406-6 (EURL GMFF, 2015). XX KW event_specific. XX OS Glycine max (soybean)- event DAS-44406-6(DAS-44406-6) XX RN [1] RP 1-99 RA European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission; RT "Event-specific Method for the Quantification of Soybean DAS-44406-6 by Real-time PCR - Validation Report and Validated Method"; RL Online Publication (2015). RX EURL_GMFF=EURL-VL-0112%20VR_Final.pdf RX EURL_GMFF=EURL-VL-01-12_VP_Final.pdf XX RN [2] RP 1-99 RT "PCR reactions set up and amplification conditions"; RL Online Publication (2015). RX PCR=QT-EVE-GM-015.pdf XX DR GMOMETHODS; QT-TAX-GM-002; XX FH Key Location/Qualifiers FH FT STS 1..99 FT /standard_name="PCR 99 bp amplicon" FT /note="Event-specific RT-PCR" FT /target="5' integration border region (IBR) between the insert of soybean event DAS-44406-6 and the soybean host genome" FT primer_bind 1..28 FT /standard_name="Primer forward: DAS-44406-5F" FT /note="TTATTGTTCTTGTTGTTTCCTCTTTAGG" FT /target="5'-host genome" FT primer_bind complement(41..69) FT /standard_name="RT-PCR probe: DAS-44406-6-5p1" FT /note="FAM-ATTCGGACCTCCATGATGACCTTACCGTT-TAMRA" FT primer_bind complement(76..99) FT /standard_name="Primer reverse: DAS-44406-5R" FT /note="CCTCAATTGCGAGCTTTCTAATTT" FT /target="insert" XX SQ Sequence 99 BP; 27 A; 14 C; 23 G; 35 T; 0 other; ttattgttct tgttgtttcc tctttaggnn nnnnnnnnnn aacggtaagg tcatcatgga 60 ggtccgaatn nnnnnaaatt agaaagctcg caattgagg 99 //