ID   QT-EVE-ZM-032; SV 0; linear; genomic DNA; STS; SYN; 92 BP.
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AC   MON-00810-6;
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DT   29-MAY-2009
DT   21-DEC-2022
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DE   Quantitative droplet digital PCR method for detection of maize event MON810 (Gatto et al., 2022). 
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KW   event_specific.
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OS   Zea mays (maize) - event MON810 (MON-00810-6) 
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RN   [1]
RP   1-92
RA   Gatto F., Savini C., Sacco MG., Vinciguerra D., Buttinger G., Corbisier P., Mazzara M., Emons H.;
RT   "Single and multi-laboratory validation of a droplet digital PCR method";
RL   Food Control, 140:109117 (2022).
RX   DOI=10.1016/j.foodcont.2022.109117
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RN   [2]
RP   1-92
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2022).
RX   PCR=QT-EVE-ZM-032.pdf
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DR   GMOMETHODS; QT-TAX-ZM-007;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..92
FT                   /standard_name="PCR 92 bp amplicon"
FT                   /note="Event-specific RT-PCR"
FT                   /target="5' integration border region (IBR) between the insert of maize event MON810 and the maize host genome"
FT   primer_bind     1..24
FT                   /standard_name="Primer forward: Mail-F1"
FT                   /note="TCGAAGGACGAAGGACTCTAACGT"
FT                   /target="5'-host genome"
FT   primer_bind     27..49
FT                   /standard_name="RT-PCR probe: Mail-S2"
FT                   /note="FAM-AACATCCTTTGCCATTGCCCAGC-TAMRA"
FT   primer_bind     complement(69..92)
FT                   /standard_name="Primer reverse: Mail-R1"
FT                   /note="GCCACCTTCCTTTTCCACTATCTT"
FT                   /target="insert"
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SQ   Sequence 92 BP; 18 A; 6 C; 17 G; 7 T; 44 other;
     tcgaaggacg aaggactcta acgtnnaaca tcctttgcca ttgcccagcn nnnnnnnnnn        60
     nnnnnnnnaa gatagtggaa aaggaaggtg gc                                      92
//