ID   QT-TAX-GH-020; SV 0; linear; genomic DNA; STS; PLN; 73 BP.
XX
AC   ;
XX
DT   04-AGO-2020
DT   04-AGO-2020
XX
DE   Quantitative PCR method for detection of cotton alcohol dehydrogenase C gene
XX
KW   taxon_specific, validated_in_combination.
XX
OS   Gossypium hirsutum (upland cotton)
XX
RN   [1]
RP   1-73
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Cotton GHB811 Using Real-time PCR - Validation Report";   
RL   Online Publication (2020).
RX   EURL_GMFF=EURL-VL-04-18-VR_GHB811.pdf
RX   EURL_GMFF=EURL-VL-04-18-VP_GHB811.pdf
XX
RN   [2]
RP   1-73
RT   "See Cross-references below";
RL   Online Publication (2020).
XX
DR   GMOMETHODS; QT-EVE-GH-013;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..73
FT                   /standard_name="PCR 73 bp amplicon"
FT                   /note="taxon-specific RT-PCR"
FT                   /target="alcohol dehydrogenase C (AdhC) gene"
FT   primer_bind     1..23
FT                   /standard_name="Primer forward: KVM157"
FT                   /note="CACATGACTTAGCCCATCTTTGC"
FT                   /target="AdhC"
FT   primer_bind     25..50
FT                   /standard_name="RT-PCR probe: TM1304"
FT                   /note="JOE-TGCAGGTTTTGGTGCCACTGTGAATG-BHQ1"
FT   primer_bind     complement(53..73)
FT                   /standard_name="Primer reverse: KVM158"
FT                   /note="CCCACCCTTTTTTGGTTTAGC"
FT                   /target="AdhC"
XX
SQ   Sequence 73 BP; 18 A; 15 C; 19 G; 18 T; 3 other;
     cacatgactt agcccatctt tgcntgcagg ttttggtgcc actgtgaatg nngctaaacc        60
     aaaaaagggt ggg                                                           73
//