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ID   QL-TAX-CP-001; SV 1; linear; genomic DNA; STD; PLN; 282 BP.
AC   ;
DT   29-NOV-2004
DT   11-MAY-2016
DE   Qualitative PCR method for detection of papaya chymopapain gene (Wei et al., 2013).
KW   Taxon_specific, validated_independently.
OS   Carica papaya (papaya)
RN   [1]
RP   1-282
RA   Wei J., Li F., Guo J., Li X., Xu J., Wu G., Zhang D., Yang L.;
RT   "Collaborative ring trial of the papaya endogenous reference gene and its
RT   polymerase chain reaction assays for genetically modified organism
RT   analysis";
RL   J. Agric. Food Chem. 61:11363-11370 (2013).
RX   DOI=10.1021/jf403338a.
RN   [2]
RP   1-282
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2016).
RX   PCR=QL-TAX-CP-001.pdf
FH   Key             Location/Qualifiers
FT   STS             1..282
FT                   /standard_name="PCR 282 bp amplicon"
FT                   /note="taxon-specific PCR for papaya"
FT                   /target="chymopapain gene"
FT   primer_bind     1..22
FT                   /standard_name="Primer forward: Chy-1F"
FT                   /note="ATCTACAATCTTGCTAACCCTA"
FT                   /target="CHY"
FT   primer_bind     complement(261..282)
FT                   /standard_name="Primer reverse: Chy-2R"
FT                   /note="AGTCATCTTGAGAATAACCCAC"
FT                   /target="CHY"
SQ   Sequence 282 BP; 84 A; 55 C; 49 G; 94 T; 0 other;
     atctacaatc ttgctaaccc taaaatgacg gtgcaagtag agtgaatgac aagatctttt        60
     atttagagag aaagaaactt gattgaaaag acaagctagt atataaaccc atgcggatcc       120
     tcccatttcc cttcatccat tcccactctt gagagttagc tagtgttaca atggctacga       180
     tgagttcaat ttcaaaaatt attttcttgg caacatgtct cattattcat atgggtttgt       240
     cttctgctga tttttacacc gtgggttatt ctcaagatga ct                          282