ID   QT-TAX-GH-016; SV 0; linear; genomic DNA; STS; PLN; 123 BP.
XX
AC   ;
XX
DT   12-JUN-2008
DT   11-DEC-2019
XX
DE   Quantitative PCR method for detection of putative cotton SAH7 protein
DE   gene (123 bp)
DE   (Mazzara et al., 2006).
XX
KW   taxon_specific, validated_in_combination.
XX
OS   Gossypium hirsutum (upland cotton)
XX
RN   [1]
RP   1-123
RA   Mazzara M., Larcher S., Savini C., Charles Delobel C., Van Den Eede G.;
RT   "Event-Specific Methods for the Quantitation of the Hybrid Cotton Line 281-24-236/3006-210-23 Using Real-Time PCR - Validation Report and Protocol - Sampling and DNA Extraction of Cotton Seeds";
RL   Online Publication (2006).
RX   DOI=10.2788/32649
XX
RN   [2]
RP   1-123
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Cotton DAS-81910-7 Using Real-time PCR - Validation Report";
RL   Online Publication (2019).
RX   EURL_GMFF=EURL-VL-06_16_VR.pdf
RX   EURL_GMFF=EURL-VL-06_16_VP.pdf
XX
RN   [3]
RP   1-123
RT   "See Cross-references below";
RL   Online Publication (2010).
XX
DR   GMOMETHODS; QT-EVE-GH-001a;
DR   GMOMETHODS; QT-EVE-GH-001b;
DR   GMOMETHODS; QT-EVE-GH-011;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..123
FT                   /standard_name="PCR 123 bp amplicon"
FT                   /note="taxon-specific RT-PCR"
FT                   /target="IVS of the putative Sinapis Arabidopsis Homolog 7 (SAH7) protein gene of Gossypium hirsutum D-subgenome"
FT                   /note="The cotton endogenous gene SAH7 (Sinapis Arabidopsis Homolog 7) is present on both cotton subgenomes A and D. Primers and probe for this cotton-specific reference system match perfectly to both subgenome gene copies but amplify a 115-bp fragment from the A-subgenome and a 123-bp fragment from the D-subgenome"
FT   primer_bind     1..28
FT                   /standard_name="Primer forward: Sah7-uni-f1"
FT                   /note="AGTTTGTAGGTTTTGATGTTACATTGAG"
FT                   /target="SAH7"
FT   primer_bind     52..84
FT                   /standard_name="RT-PCR probe: Sah7-uni-s1"
FT                   /note="FAM-AAACATAAAATAATGGGAACAACCATGACATGT-TAMRA"
FT   primer_bind     complement(103..123)
FT                   /standard_name="Primer reverse: Sah7-uni-r1"
FT                   /note="GCATCTTTGAACCGCCTACTG"
FT                   /target="SAH7"
XX
SQ   Sequence 123 BP; 29 A; 10 C; 20 G; 23 T; 41 other;
     agtttgtagg ttttgatgtt acattgagnn nnnnnnnnnn nnnnnnnnnn naaacataaa        60
     ataatgggaa caaccatgac atgtnnnnnn nnnnnnnnnn nncagtaggc ggttcaaaga       120
     tgc                                                                     123
//