ID   QT-TAX-CP-001; SV 1; linear; genomic DNA; STD; PLN; 74 BP.
XX
AC   ;
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DT   29-NOV-2004
DT   11-MAY-2016
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DE   Quantitative PCR method for detection of papaya chymopapain gene (Wei et al., 2013).
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KW   Taxon_specific, validated_independently.
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OS   Carica papaya (papaya)
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RN   [1]
RP   1-74
RA   Wei J., Li F., Guo J., Li X., Xu J., Wu G., Zhang D., Yang L.;
RT   "Collaborative ring trial of the papaya endogenous reference gene and its
RT   polymerase chain reaction assays for genetically modified organism
RT   analysis";
RL   J. Agric. Food Chem. 61:11363-11370 (2013).
RX   DOI=10.1021/jf403338a.
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RN   [2]
RP   1-74
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2016).
RX   PCR=QT-TAX-CP-001.pdf
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FH   Key             Location/Qualifiers
FH
FT   STS             1..74
FT                   /standard_name="PCR 74 bp amplicon"
FT                   /note="taxon-specific RT-PCR for papaya"
FT                   /target="chymopapain gene"
FT   primer_bind     1..17
FT                   /standard_name="Primer forward: Q-Chy-1F"
FT                   /note="CCATGCGGATCCTCCCA"
FT                   /target="CHY"
FT   primer_bind     19..46
FT                   /standard_name="RT-PCR probe: Q-Chy-P"
FT                   /note="FAM-TTCCCTTCATCCATTCCCACTCTTGAGA-3'-BHQ1"
FT   misc_feature    28
FT                   /note="To increase the fluorescent intensity, the
FT                   fluorescent quencher dye was labeled on the T-base of the
FT                   probe within internal position with black-hole quencher 1
FT                   (BHQ1)"
FT   primer_bind     complement(48..74)
FT                   /standard_name="Primer reverse: Q-Chy-2R"
FT                   /note="CATCGTAGCCATTGTAACACTAGCTAA"
FT                   /target="CHY"
XX
SQ   Sequence 74 BP; 15 A; 23 C; 13 G; 23 T; 0 other;
     ccatgcggat cctcccattt cccttcatcc attcccactc ttgagagtta gctagtgtta        60
     caatggctac gatg                                                          74
//