ID   QT-TAX-BN-012; SV 0; linear; genomic DNA; STS; PLN; 101 BP.
XX
AC   ;
XX
DT   26-JUN-2008
DT   05-DEC-2011
XX
DE   Quantitative PCR  method for detection of oilseed rape cruciferin A gene 
XX
KW   taxon_specific, validated_in_combination.
XX
OS   Brassica napus (oilseed rape)
XX
RN   [1]
RP   1-101
RA   Mazzara M., Grazioli E., Savini C., Van Den Eede G.;
RT   Event-Specific Method for the Quantification of Oilseed Rape Line RT73 Using Real-Time PCR - Validation Report and Protocol - Seeds Sampling and DNA Extraction of Oilseed Rape;
RL   Online Publication (2007).
RX   DOI=10.2788/33974
XX
RN   [2]
RP   1-101
RA   Mazzara M., Bogni A., Savini C., Van Den Eede G.;
RT   "Event-specific Method for the Quantification of Oilseed Rape Line Ms8 Using Real-time PCR - Validation Report and Protocol- Seeds Sampling and DNA Extraction of Oilseed Rape";
RL   Online Publication (2007).
RX   DOI=10.2788/33880 
XX
RN   [3]
RP   1-101
RA   Savini C., Bogni A., Mazzara M., Van Den Eede G.;
RT   "Event-specific Method for the Quantification of Oilseed Rape Line Rf3 Using Real-time PCR - Validation Report and Protocol - Seeds Sampling and DNA Extraction";
RL   Online Publication (2007).
RX   DOI=10.2788/28179 
XX
RN   [4]
RP   1-101
RA   Charles Delobel C., Bogni A., Mazzara M., Savini C., Van Den Eede G.;
RT   "Event-specific Method for the Quantification of Oilseed Rape Line T45 Using Real-time PCR - Validation Report and Protocol - Sampling and DNA Extraction of Oilseed Rape";
RL   Online Publication (2006).
RX   DOI=10.2788/30936
XX
RN   [5]
RP   1-101
RA   Savini C., ;
RT   "In-house Validation of an Event-specific Method for the Quantification of Oilseed Rape MS1 Using Real-time PCR - Validation Report and Protocol";
RL   Online Publication (2011).
RX   EURL_GMFF=CRLVL1104 VR.pdf
RX   EURL_GMFF=CRLVL1104 VP.pdf
XX
RN   [6]
RP   1-101
RA   Mazzara M.,;
RT   "In-house Validation of an Event-specific Method for the Quantification of Oilseed Rape RF1 Using Real-time PCR - Validation Report and Protocol";
RL   Online Publication (2011).
RX   EURL_GMFF=CRLVL0904 VR.pdf
RX   EURL_GMFF=CRLVL0904 VP.pdf
XX
RN   [7]
RP   1-101
RA   Mazzara M.,;
RT   "In-house Validation of an Event-specific Method for the Quantification of Oilseed Rape RF2 Using Real-time PCR - Validation Report and Protocol";
RL   Online Publication (2011).
RX   EURL_GMFF=Rf2 Validation Report.pdf
RX   EURL_GMFF=Rf2 Validated Method Protocol CRL-VL-1004.pdf
XX
RN   [8]
RP   1-101
RA   Mazzara M.,;
RT   "In-house Validation of an Event-specific Method for the Quantification of Oilseed Rape Topas 19/2 Using Real-time PCR - Validation Report and Protocol";
RL   Online Publication (2011).
RX   EURL_GMFF=CRLVL1204 VR.pdf
RX   EURL_GMFF=CRLVL1204 VP.pdf
XX
RN   [9]
RP   1-101
RT   "See Cross-references below";
RL   Online Publication (2010).
XX
DR   GMOMETHODS; QT-EVE-BN-004;
DR   GMOMETHODS; QT-EVE-BN-002;
DR   GMOMETHODS; QT-EVE-BN-003;
DR   GMOMETHODS; QT-EVE-BN-001;
DR   GMOMETHODS; QT-EVE-BN-005;
DR   GMOMETHODS; QT-EVE-BN-006;
DR   GMOMETHODS; QT-EVE-BN-007;
DR   GMOMETHODS; QT-EVE-BN-008;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             complement(1..101)
FT                   /standard_name="PCR 101 bp amplicon"
FT                   /note="taxon-specific RT-PCR for oilseed rape"
FT                   /target="cruciferin A (CruA) gene"
FT   primer_bind     1..18
FT                   /standard_name="Primer forward: MDB510"
FT                   /note="GGCCAGGGTTTCCGTGAT"
FT                   /target="CruA"
FT   primer_bind     complement(20..48)
FT                   /standard_name="RT-PCR probe: TM003"
FT                   /note="VIC-AGTCCTTATGTGCTCCACTTTCTGGTGCA-TAMRA"
FT   primer_bind     complement(81..101)
FT                   /standard_name="Primer reverse: MDB511"
FT                   /note="CCGTCGTTGTAGAACCATTGG"
FT                   /target="CruA"
XX
SQ   Sequence 101 BP; 19 A; 16 C; 20 G; 13 T; 33 other;
     ggccagggtt tccgtgatnt gcaccagaaa gtggagcaca taaggactnn nnnnnnnnnn        60
     nnnnnnnnnn nnnnnnnnnn ccaatggttc tacaacgacg g                           101
//