ID QL-ELE-00-030; SV 0; linear; genomic DNA; STD; SYN; 197 BP. XX AC ; XX DT 17-AUG-2009 DT 12-DEC-2017 XX DE Qualitative LAMP method for detection of Cry1Ac gene (Li et al., 2018). XX KW element_specific. XX RN [1] RP 1-197 RA Li R., Shi J., Liu B., Zhang D., Zhao X., Yang L.; RT "International collaborative ring trial of four gene-specific loop-mediated isothermal amplification assays in GMO analysis"; RL Food Control 84:278-283 (2018). RX DOI=10.1016/j.foodcont.2017.08.012 XX RN [2] RP 1-197 RA Wang C., Li R., Quan S., Shen P., Zhang D., Shi J., Yang L.; RT "GMO detection in food and feed through screening by visual loop-mediated isothermal amplification assays"; RL Anal. and Bioanal. Chem. 407:4829-4834(2015). RX DOI=10.1007/s00216-015-8652-z XX RN [3] RP 1-197 RT "PCR reactions set up and amplification conditions"; RL Online Publication (2017). RX PCR=QL-ELE-00-030.pdf XX FH Key Location/Qualifiers FH FT STS 1..197 FT /standard_name="LAMP 197 bp amplicon" FT /note="element-specific LAMP assay" FT /target="Cry1Ac modified gene derived from Bacillus thuringiensis" FT primer_bind 1..18 FT /standard_name="Primer forward: cry1Ac F3" FT /note="GAGGCCAAAGAGTCCGTG" FT /target="cry1Ac" FT primer_bind join(21..41,complement(68..87)) FT /standard_name="Primer: cry1Ac FIP" FT /note="GGCGTGGATCATGGCGATGTTGCTTTGTTCGGGAACTCCC FT A" FT primer_bind 21..41 FT /note="TGCTTTGTTCGGGAACTCCCA" FT /target="cry1Ac F2 (in position 32 the sequence of the FT amplicon includes an oligonucleotide T instead of FT oligonucleotide G of the primer sequence)" FT primer_bind complement(43..65) FT /standard_name="Primer: cry1Ac loop F (complementary to the sequence between F1 and F2)" FT /note="GTGTCGGCTTGCAACTGATCATA" FT primer_bind complement(68..87) FT /note="GGCGTGGATCATGGCGATGT" FT /target="cry1Ac F1c (complementary to F1)" FT primer_bind join(96..117,complement(157..174)) FT /standard_name="Primer: cry1Ac BIP" FT /note="ACGTGTGCACAGCATTCGTGAGTTCCTCGAAGATGGCAGC FT " FT primer_bind 96..117 FT /note="ACGTGTGCACAGCATTCGTGAG" FT /target="cry1Ac B1c (complementary to B1)" FT primer_bind 127..148 FT /standard_name="Primer: cry1Ac loop B (complementary to the sequence between B1 and B2)" FT /note="CCTGAGTTGTCCGTGATCCCTG" FT primer_bind complement(157..174) FT /note="TTCCTCGAAGATGGCAGC" FT /target="cry1Ac B2" FT primer_bind complement(178..197) FT /standard_name="Primer reverse: cry1Ac B3" FT /note="GCGGTAAAGATACGTCCCTC" FT /target="cry1Ac" XX SQ Sequence 197 BP; 45 A; 54 C; 51 G; 47 T; 0 other; gaggccaaag agtccgtgnn tgctttgttc gtgaactccc antatgatca gttgcaagcc 60 gacacnnaca tcgccatgat ccacgccnnn nnnnnacgtg tgcacagcat tcgtgagnnn 120 nnnnnncctg agttgtccgt gatccctgnn nnnnnngctg ccatcttcga ggaannngag 180 ggacgtatct ttaccgc 197 //