Only one hit for query 'ac%3aBCS-GH005-8'
ID QL-ELE-00-030; SV 0; linear; genomic DNA; STD; SYN; 197 BP.
XX
AC ;
XX
DT 17-AUG-2009
DT 12-DEC-2017
XX
DE Qualitative LAMP method for detection of Cry1Ac gene (Li et al., 2018).
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KW element_specific.
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RN [1]
RP 1-197
RA Li R., Shi J., Liu B., Zhang D., Zhao X., Yang L.;
RT "International collaborative ring trial of four gene-specific loop-mediated isothermal amplification assays in GMO analysis";
RL Food Control 84:278-283 (2018).
RX DOI=10.1016/j.foodcont.2017.08.012
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RN [2]
RP 1-197
RA Wang C., Li R., Quan S., Shen P., Zhang D., Shi J., Yang L.;
RT "GMO detection in food and feed through screening by visual loop-mediated isothermal amplification assays";
RL Anal. and Bioanal. Chem. 407:4829-4834(2015).
RX DOI=10.1007/s00216-015-8652-z
XX
RN [3]
RP 1-197
RT "PCR reactions set up and amplification conditions";
RL Online Publication (2017).
RX PCR=QL-ELE-00-030.pdf
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FH Key Location/Qualifiers
FH
FT STS 1..197
FT /standard_name="LAMP 197 bp amplicon"
FT /note="element-specific LAMP assay"
FT /target="Cry1Ac modified gene derived from Bacillus thuringiensis"
FT primer_bind 1..18
FT /standard_name="Primer forward: cry1Ac F3"
FT /note="GAGGCCAAAGAGTCCGTG"
FT /target="cry1Ac"
FT primer_bind join(21..41,complement(68..87))
FT /standard_name="Primer: cry1Ac FIP"
FT /note="GGCGTGGATCATGGCGATGTTGCTTTGTTCGGGAACTCCC
FT A"
FT primer_bind 21..41
FT /note="TGCTTTGTTCGGGAACTCCCA"
FT /target="cry1Ac F2 (in position 32 the sequence of the
FT amplicon includes an oligonucleotide T instead of
FT oligonucleotide G of the primer sequence)"
FT primer_bind complement(43..65)
FT /standard_name="Primer: cry1Ac loop F (complementary to the sequence between F1 and F2)"
FT /note="GTGTCGGCTTGCAACTGATCATA"
FT primer_bind complement(68..87)
FT /note="GGCGTGGATCATGGCGATGT"
FT /target="cry1Ac F1c (complementary to F1)"
FT primer_bind join(96..117,complement(157..174))
FT /standard_name="Primer: cry1Ac BIP"
FT /note="ACGTGTGCACAGCATTCGTGAGTTCCTCGAAGATGGCAGC
FT "
FT primer_bind 96..117
FT /note="ACGTGTGCACAGCATTCGTGAG"
FT /target="cry1Ac B1c (complementary to B1)"
FT primer_bind 127..148
FT /standard_name="Primer: cry1Ac loop B (complementary to the sequence between B1 and B2)"
FT /note="CCTGAGTTGTCCGTGATCCCTG"
FT primer_bind complement(157..174)
FT /note="TTCCTCGAAGATGGCAGC"
FT /target="cry1Ac B2"
FT primer_bind complement(178..197)
FT /standard_name="Primer reverse: cry1Ac B3"
FT /note="GCGGTAAAGATACGTCCCTC"
FT /target="cry1Ac"
XX
SQ Sequence 197 BP; 45 A; 54 C; 51 G; 47 T; 0 other;
gaggccaaag agtccgtgnn tgctttgttc gtgaactccc antatgatca gttgcaagcc 60
gacacnnaca tcgccatgat ccacgccnnn nnnnnacgtg tgcacagcat tcgtgagnnn 120
nnnnnncctg agttgtccgt gatccctgnn nnnnnngctg ccatcttcga ggaannngag 180
ggacgtatct ttaccgc 197
//