ID   QT-EVE-ZM-039; SV 0; linear; genomic DNA; STD; PLN; 80 BP.
XX
AC   DP-051291-2;
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DT   12-JUN-2024
DT   12-JUN-2024
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DE   Quantitative PCR method for detection of maize event DP51291 (EURL GMFF, 2024).
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KW   event_specific.
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OS   Zea mays (maize) - event DP51291 (DP-051291-2)
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RN   [1]
RP   1-80
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Maize event DP51291 Using Real-time PCR - Validation Report"; 
RL   Online Publication (2024).
RX   EURL_GMFF=EURL-VL-04-22-VR.pdf
RX   EURL_GMFF=EURL-VL-04-22-VM.pdf
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RN   [2]
RP   1-80
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2024).
RX   PCR=QT-EVE-ZM-039.pdf
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DR   GMOMETHODS; QT-TAX-ZM-008;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..80
FT                   /standard_name="PCR 80 bp amplicon"
FT                   /note="Event-specific RT-PCR"
FT                   /target="3' integration border region (IBR) between the insert of maize event DP51291 and the maize host genome"
FT   primer_bind     1..27
FT                   /standard_name="Primer forward: DP-051291-2 Forward Primer"
FT                   /note="CGCAATGTGTTATTAAGTTGTCTAAGC"
FT                   /target="insert"
FT   primer_bind     33..53
FT                   /standard_name="RT-PCR probe: DP-051291-2 Probe"
FT                   /note="FAM-TTTGTTTACACCACATACACG-MGBNFQ"
FT   primer_bind     complement(59..80)
FT                   /standard_name="Primer reverse: DP-051291-2 Reverse Primer"
FT                   /note="CACGGTTAGCTGTACCAAATGC"
FT                   /target="3'-host genome"
XX
SQ   Sequence 80 BP; 22 A; 18 C; 15 G; 25 T; 0 other;
     cgcaatgtgt tattaagttg tctaagcnnn nntttgttta caccacatac acgnnnnngc        60
     atttggtaca gctaaccgtg                                                    80
//