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ID   QL-ELE-00-004; SV 0; linear; genomic DNA; STS; SYN; 123 BP.
AC   ;
DT   23-JUN-2009
DT   04-OCT-2010
DE   Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter
DE   (Lipp et al.,2001).
KW   element_specific.
RN   [1]
RP   1-123
RA   Lipp M., Bluth A., Eyquem F., Kruse L., Schimmel H., Van den Eede G.,
RA   Anklam E.;
RT   "Validation of a method based on polymerase chain reaction for the
RT   detection of genetically modified organisms in various processed
RT   foodstuffs";
RL   Eur. Food Res. Technol. 212:497-504 (2001).
RX   DOI=10.1007/s002170000274
RN   [2]
RP   1-123
RT   "Foodstuffs - Methods of analysis for the detection of genetically
RT   modified organisms and derived products - Qualitative nucleic acid based
RT   methods";
RL   ISO 21569:1-69 (2005).
RX   ISO=34614
RN   [3]
RP   1-123
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QL-ELE-00-004.pdf
FH   Key             Location/Qualifiers
FT   STS             1..123
FT                   /standard_name="PCR 123 bp amplicon"
FT                   /note="element-specific PCR"
FT                   /target="Cauliflower Mosaic Virus 35S promoter (CaMV P-35S)"
FT   primer_bind     1..21
FT                   /standard_name="Primer forward: 35S-cf3"
FT                   /note="CCACGTCTTCAAAGCAAGTGG"
FT                   /target="CaMV P-35S"
FT   primer_bind     complement(99..123)
FT                   /standard_name="Primer reverse: 35S-cr4"
FT                   /note="TCCTCTCCAAATGAAATGAACTTCC"
FT                   /target="CaMV P-35S"
SQ   Sequence 123 BP; 13 A; 8 C; 13 G; 12 T; 77 other;
     ccacgtcttc aaagcaagtg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg aagttcattt catttggaga       120
     gga                                                                     123