ID   QT-EVE-ZM-038; SV 0; linear; genomic DNA; STD; PLN; 108 BP.
XX
AC   DP-910521-2;
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DT   07-JUN-2024
DT   07-JUN-2024
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DE   Quantitative PCR method for detection of maize event DP910521 (EURL GMFF, 2024).
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KW   event_specific.
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OS   Zea mays (maize) - event DP910521 (DP-910521-2)
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RN   [1]
RP   1-108
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Maize DP910521 Using Real-time PCR - Validation Report";
RL   Online Publication (2024).
RX   EURL_GMFF=EURL-VL-04-21-VR.pdf
RX   EURL_GMFF=EURL-VL-04-21-VM.pdf
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RN   [2]
RP   1-108
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2024).
RX   PCR=QT-EVE-ZM-038.pdf
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DR   GMOMETHODS; QT-TAX-ZM-008;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..108
FT                   /standard_name="PCR 108 bp amplicon"
FT                   /note="Event-specific RT-PCR"
FT                   /target="5' integration border region (IBR) between the insert of maize event DP910521 and the maize host genome"                
FT   primer_bind     1..25
FT                   /standard_name="Primer forward: PHN201324"
FT                   /note="CTCTTGACACTTTGTATTGGTGCTC"
FT                   /target="5'-host genome"
FT   primer_bind     26..42
FT                   /standard_name="RT-PCR probe: PHN201325"
FT                   /note="FAM-TTGGGCTCAAGAGGGTA-MGBNFQ"
FT   primer_bind     complement(87..108)
FT                   /standard_name="Primer reverse: PHN165631"
FT                   /note="CATAGTAACCGTGAGCGCTTCA"
FT                   /target="insert"
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SQ   Sequence 108 BP; 24 A; 25 C; 27 G; 32 T; 0 other;
     ctcttgacac tttgtattgg tgctcttggg ctcaagaggg tannnnnnnn nnnnnnnnnn        60
     nnnnnnnnnn nnnnnnnnnn nnnnnntgaa gcgctcacgg ttactatg                    108
//