ID   QT-EVE-ZM-037; SV 0; linear; genomic DNA; STD; SYN; 103 BP.
XX
AC   MON-94804-4;
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DT   06-JUN-2024
DT   06-JUN-2024
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DE   Quantitative PCR method for detection of maize event MON 94804 (EURL GMFF, 2024).
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KW   event_specific.
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OS   Zea mays (maize) - event MON 94804 (MON-94804-4)
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RN   [1]
RP   1-103
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Maize MON 94804 Using
RT   Real-time PCR - Validation Report";
RL   Online Publication (2024).
RX   EURL_GMFF=EURL-VL-06-22-VR.pdf
RX   EURL_GMFF=EURL-VL-06-22-VM.pdf
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RN   [2]
RP   1-103
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2024).
RX   PCR=QT-EVE-ZM-037.pdf
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DR   GMOMETHODS; QT-TAX-ZM-002;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..103
FT                   /standard_name="PCR 103 bp amplicon"
FT                   /note="Event-specific RT-PCR"
FT                   /target="5' integration border region (IBR) between the insert of maize event MON 94804 and the maize host genome"
FT   primer_bind     1..21
FT                   /standard_name="Primer forward: MON 94804 primer 1"
FT                   /note="CTCTTCTAATCCGGGCCATCG"
FT                   /target="5'-host genome"
FT   primer_bind     25..53
FT                   /standard_name="RT-PCR probe: MON 94804 probe"
FT                   /note="FAM-CTGGATCCGAAGGACGTGTCTACATTCAC-TAMRA"
FT   primer_bind     complement(83..103)
FT                   /standard_name="Primer reverse: MON 94804 primer 2"
FT                   /note="AGTTAGTCGCGCCAAATCGTG"
FT                   /target="insert"
XX
SQ   Sequence 103 BP; 25 A; 27 C; 26 G; 25 T; 0 other;
     ctcttctaat ccgggccatc gnnnctggat ccgaaggacg tgtctacatt cacnnnnnnn        60
     nnnnnnnnnn nnnnnnnnnn nncacgattt ggcgcgacta act                         103
//