ID   QT-EVE-ZM-033; SV 0; linear; genomic DNA; STD; PLN; 87 BP.
XX
AC   MON-95379-3;
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DT   20-DEC-2022
DT   20-DEC-2022
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DE   Quantitative PCR method for detection of maize event MON 95379 (EURL GMFF, 2022).
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KW   event_specific.
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OS   Zea mays (maize) - event MON 95379 (MON-95379-3)
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RN   [1]
RP   1-87
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT  "Event-specific Method for the Quantification of Maize MON 95379 Using Real-time PCR - Validation Report"; 
RL   Online Publication (2022).
RX   EURL_GMFF=EURL-VL-06-20-VR.pdf
RX   EURL_GMFF=EURL-VL-06-20-VM.pdf
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RN   [2]
RP   1-87
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2022).
RX   PCR=QT-EVE-ZM-033.pdf
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DR   GMOMETHODS; QT-TAX-ZM-002;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..87
FT                   /standard_name="PCR 87 bp amplicon"
FT                   /note="Event-specific RT-PCR"
FT                   /target="3' integration border region (IBR) between the insert of maize event MON 95379 and the maize host genome"
FT   primer_bind     1..22
FT                   /standard_name="Primer forward: MON 95379 primer 1"
FT                   /note="CCAAGAAGAACGATTGGCAAAC"
FT                   /target="insert"
FT   primer_bind     30..62
FT                   /standard_name="RT-PCR probe: MON 95379 probe"
FT                   /note="FAM-ATGGGTATTATGGGTAGGCACATGGGAATATAG-TAMRA"
FT   primer_bind     complement(70..87)
FT                   /standard_name="Primer reverse: MON 95379 primer 2"
FT                   /note="GGCACAGGCACGCCTCTG"
FT                   /target="3'-host genome"
XX
SQ   Sequence 87 BP; 25 A; 14 C; 30 G; 18 T; 0 other;
     ccaagaagaa cgattggcaa acnnnnnnna tgggtattat gggtaggcac atgggaatat        60
     agnnnnnnnc agaggcgtgc ctgtgcc                                            87
//