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ID   QL-ELE-00-031; SV 0; linear; genomic DNA; STD; SYN; 184 BP.
XX
AC   ;
XX
DT   01-APR-2009
DT   07-DEC-2018
XX
DE   Qualitative LAMP method for detection of Figwort Mosaic Virus 35S promoter (Li et al., 2019).
XX
KW   element_specific.
XX
RN   [1]
RP   1-184
RA   Li R., Shi J., Liu B., Wang C., Zhang D., Zhao X.;
RT   "Inter-laboratory validation of visual loop-mediated isothermal
RT   amplification assays for GM contents screening";
RL   Food Chemistry 274:659-663 (2019).
RX   DOI=10.1016/j.foodchem.2018.07.010
XX
RN   [2]
RP   1-184
RA   Wang C., Li R., Quan S., Shen P., Zhang D., Shi J., Yang L.;
RT   "GMO detection in food and feed through screening by visual loop-mediated isothermal amplification assays";
RL   Anal. and Bioanal. Chem.  407:4829-4834(2015).
RX   DOI=10.1007/s00216-015-8652-z
XX
RN   [3]
RP   1-184
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2018).
RX   PCR=QL-ELE-00-031.pdf
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..184
FT                   /standard_name="LAMP 184 bp amplicon"
FT                   /note="element-specific LAMP assay"
FT                   /target="Figwort Mosaic Virus 35S promoter (P-FMV)"
FT   primer_bind     1..19
FT                   /standard_name="Primer forward: P-FMV35S F3"
FT                   /note="GTCAGGGTACAGAGTCTCC"
FT                   /target="P-FMV"
FT   primer_bind     join(complement(66..87),22..41)
FT                   /standard_name="Primer: P-FMV35S FIP"
FT                   /note="GCTGGAACAGTAGTTTACTTTGACCATTAGCCAAAAGCTACA"
FT   primer_bind     22..41
FT                   /note="ACCATTAGCCAAAAGCTACA"
FT                   /target="P-FMV35S F2"
FT   primer_bind     complement(42..65)
FT                   /standard_name="Primer: P-FMV35S loop F (complementary to the sequence between F1 and F2)"
FT                   /note="ATTGAAGATTCTTCATTGATCTCC"
FT   primer_bind     complement(66..87)
FT                   /note="GCTGGAACAGTAGTTTACTTTG"
FT                   /target="P-FMV35S F1c (complimentary to
FT                   F1)"
FT   primer_bind     join(88..105,complement(145..168))
FT                   /standard_name="Primer: P-FMV35S BIP"
FT                   /note="ACATGCATGGTCAGTAAGATTACTTTCAAAGATGCCCAC"
FT   primer_bind     88..105
FT                   /note="ACATGCATGGTCAGT"
FT                   /target="P-FMV35S B1c (complementary to
FT                   B1) (according to the sequence of the FMV genome the BIP primer is missing the TCA
FT                   nucleotides in position 96-98 of the amplicon)"
FT   primer_bind     108..144
FT                   /standard_name="Primer: P-FMV35S loop B (complementary
FT                   to the sequence between B1 and B2)"
FT                   /note="GTTTCAGAAAAAGACATCCACCGAAGACTTAAAGTTA"
FT   primer_bind     complement(145..168)
FT                   /note="AAGATTACTTTCAAAGATGCCCAC"
FT                   /target="P-FMV35S B2"
FT   primer_bind     complement(167..184)
FT                   /standard_name="Primer reverse: P-FMV35S B3"
FT                   /note="GCTGCTCGATGTTGACAA"
FT                   /target="P-FMV"
XX
SQ   Sequence 184 BP; 67 A; 39 C; 36 G; 42 T; 0 other;
     gtcagggtac agagtctccn naccattagc caaaagctac aggagatcaa tgaagaatct        60
     tcaatcaaag taaactactg ttccagcaca tgcatcatgg tcagtnngtt tcagaaaaag       120
     acatccaccg aagacttaaa gttagtgggc atctttgaaa gtaatcttgt caacatcgag       180
     cagc                                                                    184
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