Only one hit for query 'ac%3AMON-89034-3'
ID QL-ELE-00-004; SV 0; linear; genomic DNA; STS; SYN; 123 BP.
XX
AC ;
XX
DT 23-JUN-2009
DT 04-OCT-2010
XX
DE Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter
DE (Lipp et al.,2001).
XX
KW element_specific.
XX
RN [1]
RP 1-123
RA Lipp M., Bluth A., Eyquem F., Kruse L., Schimmel H., Van den Eede G.,
RA Anklam E.;
RT "Validation of a method based on polymerase chain reaction for the
RT detection of genetically modified organisms in various processed
RT foodstuffs";
RL Eur. Food Res. Technol. 212:497-504 (2001).
RX DOI=10.1007/s002170000274
XX
RN [2]
RP 1-123
RT "Foodstuffs - Methods of analysis for the detection of genetically
RT modified organisms and derived products - Qualitative nucleic acid based
RT methods";
RL ISO 21569:1-69 (2005).
RX ISO=34614
XX
RN [3]
RP 1-123
RT "PCR reactions set up and amplification conditions";
RL Online Publication (2010).
RX PCR=QL-ELE-00-004.pdf
XX
FH Key Location/Qualifiers
FH
FT STS 1..123
FT /standard_name="PCR 123 bp amplicon"
FT /note="element-specific PCR"
FT /target="Cauliflower Mosaic Virus 35S promoter (CaMV P-35S)"
FT primer_bind 1..21
FT /standard_name="Primer forward: 35S-cf3"
FT /note="CCACGTCTTCAAAGCAAGTGG"
FT /target="CaMV P-35S"
FT primer_bind complement(99..123)
FT /standard_name="Primer reverse: 35S-cr4"
FT /note="TCCTCTCCAAATGAAATGAACTTCC"
FT /target="CaMV P-35S"
XX
SQ Sequence 123 BP; 13 A; 8 C; 13 G; 12 T; 77 other;
ccacgtcttc aaagcaagtg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 60
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg aagttcattt catttggaga 120
gga 123
//