ID   QT-EVE-ZM-034; SV 0; linear; genomic DNA; STD; SYN; 105 BP.
XX
AC   DP-202216-6;
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DT   21-JUL-2023
DT   21-JUL-2023
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DE   Quantitative PCR method for detection of maize event DP202216 (EURL GMFF, 2023).
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KW   event_specific.
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OS   Zea mays (maize) - event DP202216 (DP-202216-6)
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RN   [1]
RP   1-105
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Maize DP202216 Using
RT   Real-time PCR - Validation Report";
RL   Online Publication (2023).
RX   EURL_GMFF=EURL-VL-03-19-VR.pdf
RX   EURL_GMFF=EURL-VL-03-19-VM.pdf
XX
RN   [2]
RP   1-105
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2023).
RX   PCR=QT-EVE-ZM-034.pdf
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DR   GMOMETHODS; QT-TAX-ZM-008;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..105
FT                   /standard_name="PCR 105 bp amplicon"
FT                   /note="Event-specific RT-PCR"
FT                   /target="5' integration border region (IBR) between the insert of maize event DP202216 and the maize host genome"                
FT   primer_bind     1..23
FT                   /standard_name="Primer forward: PHN165665"
FT                   /note="CCATCTGAGGTCTGCACTCTCAC"
FT                   /target="5'-host genome"
FT   primer_bind     41..62
FT                   /standard_name="RT-PCR probe: PHN-2074 Probe 1"
FT                   /note="FAM-CAACACACTCAAACACTGATAG-MGBNFQ"
FT   primer_bind     complement(84..105)
FT                   /standard_name="Primer reverse: PHN143487"
FT                   /note="CTCCGCTCATGATCAGATTGTC"
FT                   /target="insert"
XX
SQ   Sequence 105 BP; 36 A; 27 C; 23 G; 19 T; 0 other;
     ccatctgagg tctgcactct cacnnnnnnn nnnnnnnnnn caacacactc aaacactgat        60
     agnnnnnnnn nnnnnnnnnn nnngacaatc tgatcatgag cggag                       105
//