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ID   QT-TAX-OS-002; SV 0; linear; mRNA; STD; PLN; 124 BP.
XX
AC   ;
XX
DT   09-JUN-2008
DT   08-APR-2016
XX
DE   Quantitative PCR method for detection of rice phospholipase D alpha 2 gene  (Jacchia et al., 2015)
XX
KW   Taxon_specific , validated_in_combination.
XX
OS   Oryza sativa (rice) 
XX
RN   [1]
RP   1-124
RA   Jacchia S., Nardini E., Bassani N., Savini C., Shim J-H., Trijatmiko K., Kreysa J., Mazzara M.;
RT   " International ring trial for the validation of an event-specific Golden Rice 2 quantitative real-time polymerase chain reaction method";
RL   J. Agric. Food Chem. 63:4954-4965 (2015).
RX   DOI=10.1021/acs.jafc.5b00951
XX
RN   [2]
RP   1-124
RA   Jacchia S., Nardini E., Savini C., Petrillo M., Angers-Loustau A., Shim J-H., Trijatmiko K., Kreysa J., Mazzara M.;
RT   " Development, optimization, and single laboratory validation of an event-specific real-time PCR method for the detection and quantification of Golden Rice 2 using a novel taxon-specific assay";
RL   J. Agric. Food Chem. 63:1711-1721 (2015).
RX   DOI=10.1021/jf505516y
XX
RN   [3]
RP   1-124
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2016).
RX   PCR=QT-TAX-OS-002.pdf
XX
DR   GMOMETHODS; QT-EVE-OS-001;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..124
FT                   /standard_name="PCR 124 bp amplicon"
FT                   /note="taxon-specific RT-PCR for rice"
FT                   /target="phospholipase D alpha 2 (PLD) gene"
FT   primer_bind     1..22
FT                   /standard_name="Primer forward: PLD-GR For"
FT                   /note="GGCAAGAACAACACCATTGACA"
FT                   /target="PLD ?2"
FT   primer_bind     52..73
FT                   /standard_name="RT-PCR probe: PLD-GR Probe"
FT                   /note="FAM-ATTCGCCGCGCCAAGAACTTCA-TAMRA"
FT   primer_bind     complement(106..124)
FT                   /standard_name="Primer reverse: PLD-GR Rev"
FT                   /note="CGGCTTTCCATGCAAATGA"
FT                   /target="PLD ?2"
XX
SQ   Sequence 124 BP; 39 A; 36 C; 25 G; 24 T; 0 other;
     ggcaagaaca acaccattga cannnnnnnn nnnnnnnnnn nnnnnnnnnn nattcgccgc        60
     gccaagaact tcannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcatt tgcatggaaa       120
     gccg                                                                    124
//