ID   QT-EVE-ZM-020; SV 0; linear; genomic DNA; STS; SYN; 92 BP.
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AC   MON-00810-6;
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DT   29-MAY-2006
DT   10-JAN-2023
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DE   Quantitative PCR method for detection of maize event MON810.
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KW   event_specific.
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OS   Zea mays (maize) - event MON810 (MON-00810-6)
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RN   [1]
RP   1-92
RT   "Foodstuffs - Methods of analysis for the detection of genetically
RT   modified organisms and derived products - Quantitative nucleic acid based
RT   methods";
RL   ISO 21570:1-103 (2005).
RX   ISO=34615
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RN   [2]
RP   1-92
RA   Mazzara M., Grazioli E., Savini C., Van Den Eede G.;
RT   "Report on the Verification of the Performance of a MON810 Event-specific Method on Maize Line MON810 Using Real-time PCR - Validation Report and Protocol";
RL   Online Publication (2009).
RX   DOI=10.2788/59036
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RN   [3]
RP   1-92
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QT-EVE-ZM-020.pdf
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DR   GMOMETHODS; QT-TAX-ZM-002;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..92
FT                   /standard_name="PCR 92 bp amplicon"
FT                   /note="Event-specific RT-PCR"
FT                   /target="5' integration border region (IBR) between the insert of maize event MON810 and the maize host genome"
FT   primer_bind     1..24
FT                   /standard_name="Primer forward: Mail-F1"
FT                   /note="TCGAAGGACGAAGGACTCTAACGT"
FT                   /target="5'-host genome"
FT   primer_bind     27..49
FT                   /standard_name="RT-PCR probe: Mail-S2"
FT                   /note="FAM-AACATCCTTTGCCATTGCCCAGC-TAMRA"
FT   primer_bind     complement(69..92)
FT                   /standard_name="Primer reverse: Mail-R1"
FT                   /note="GCCACCTTCCTTTTCCACTATCTT"
FT                   /target="insert"
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SQ   Sequence 92 BP; 26 A; 18 C; 23 G; 25 T; 0 other;
     tcgaaggacg aaggactcta acgtnnaaca tcctttgcca ttgcccagcn nnnnnnnnnn        60
     nnnnnnnnaa gatagtggaa aaggaaggtg gc                                      92
//