ID   QT-EVE-ZM-025; SV 0; linear; genomic DNA; STS; SYN; 88 BP.
XX
AC   MON-87403-1;
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DT   24-JUN-2015
DT   07-MAY-2018
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DE   Quantitative PCR method for detection of maize event MON 87403 (EURL GMFF, 2018).
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KW   event_specific.
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OS   Zea mays (maize) - event MON87403 (MON-87403-1)
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RN   [1]
RP   1-88
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Maize MON 87403 Using Real-time PCR - Validation Report";
RL   Online Publication (2018).
RX   EURL_GMFF=EURL-VL-02-15-VM.pdf
RX   EURL_GMFF=EURL-VL-02-15-VR.pdf
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RN   [2]
RP   1-88
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2018).
RX   PCR=QT-EVE-ZM-025.pdf
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DR   GMOMETHODS; QT-TAX-ZM-002;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..88
FT                   /standard_name="PCR 88 bp amplicon"
FT                   /note="Event-specific RT-PCR";
FT                   /target="3' integration border region (IBR) between the insert of maize event MON87403 and the maize host genome";
FT   primer_bind     1..30
FT                   /standard_name="Primer forward: 87403 QF"
FT                   /note="CTTTCTTTTTCTCCATATTGACCATCATAC"
FT                   /target="insert"
FT   primer_bind     31..58
FT                   /standard_name="RT-PCR probe: 87403 QP"
FT                   /note="FAM-TCATTGCGATCCACATTTCCCTACATGG-TAMRA"
FT   primer_bind     complement(64..88)
FT                   /standard_name="Primer reverse: 87403 QR"
FT                   /note="TACTCCGGAATGAGTGCTCTGTATC"
FT                   /target="3'-host genome"
XX
SQ   Sequence 88 BP; 21 A; 24 C; 14 G; 29 T; 0 other;
     ctttcttttt ctccatattg accatcatac tcattgcgat ccacatttcc ctacatggnn        60
     nnngatacag agcactcatt ccggagta                                           88
//