ID   QT-EVE-ZM-028; SV 0; linear; genomic DNA; STD; UNA; 73 BP.
XX
AC   SYN-00098-3;
XX
DT   23-MAY-2017
DT   19-DEC-2018
XX
DE   Quantitative PCR method for detection of maize event MZIR098 (EURL GMFF, 2018).
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KW   event_specific.
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OS   Zea mays (maize) - event MZIR098 (SYN-00098-3).
XX
RN   [1]
RP   1-73
RA   European Union Reference Laboratory for GM Food and Feed (EURL GMFF), Joint Research Centre (JRC), European Commission;
RT   "Event-specific Method for the Quantification of Maize MZIR098 Using Real-time PCR - Validation Report";
RL   Online Publication (2018).
RX   EURL_GMFF=EURL-VL-04-17-VR.pdf
RX   EURL_GMFF=EURL-VL-04-17-VM.pdf
XX
RN   [2]
RP   1-73
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2018).
RX   PCR=QT-EVE-ZM-028.pdf
XX
DR   GMOMETHODS; QT-TAX-ZM-014;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..73
FT                   /standard_name="PCR 73 bp amplicon"
FT                   /note="Event-specific RT-PCR";
FT                   /target="5' integration border region (IBR) between the insert of maize event MZIR098 and the maize host genome";
FT   primer_bind     1..23
FT                   /standard_name="Primer forward: MZIR098 forward primer"
FT                   /note="ATCTCAGACACCAAACCGAGATC"
FT                   /target="5'-host genome"
FT   primer_bind     24..49
FT                   /standard_name="RT-PCR probe: MZIR098 probe"
FT                   /note="FAM-CAAGTGACAGCGAACGGAGCTGGTTT-BHQ1"
FT   primer_bind     complement(52..73)
FT                   /standard_name="Primer reverse: MZIR098 reverse primer"
FT                   /note="ACACCGTTAGGCTAGTGCCAGT"
FT                   /target="insert"
XX
SQ   Sequence 73 BP; 23 A; 19 C; 18 G; 13 T; 0 other;
     atctcagaca ccaaaccgag atccaagtga cagcgaacgg agctggtttn nactggcact        60
     agcctaacgg tgt                                                           73
//