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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)


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ID   QT-EVE-OS-002; SV 0; linear; genomic DNA; STS; SYN; 88 BP.
XX
AC   ACS-OS002-5;
XX
DT   22-JAN-2007
DT   17-NOV-2010
XX
DE   Quantitative PCR method for detection of rice event LLRICE62
DE   (Mazzara et al., 2006).
XX
KW   event_specific.
XX
OS   Oryza sativa (rice) - event LLRICE62 (ACS-OS002-5)
XX
RN   [1]
RP   1-88
RA   Mazzara M., Grazioli E., Savini C., Van Den Eede G.;
RT   "Event-specific Method for the Quantitation of Rice Line LLRICE62 Using Real-time PCR -Validation Report and Protocol - Sampling and DNA Extraction of Rice";
RL   Online Publication (2006).
RX   DOI=10.2788/31940
XX
RN   [2]
RP   1-88
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QT-EVE-OS-002.pdf
XX
DR   GMOMETHODS; QT-TAX-OS-017;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..88
FT                   /standard_name="PCR 88 bp_amplicon"
FT                   /note="event-specific RT-PCR"
FT                   /target="3' integration border region (IBR) between the insert of rice event LLRICE62 and the rice host genome"
FT   primer_bind     1..22
FT                   /standard_name="Primer forward: MDB616"
FT                   /note="AGCTGGCGTAATAGCGAAGAGG"
FT                   /target="insert"
FT   primer_bind     25..54
FT                   /standard_name="RT-PCR probe: TM019"
FT                   /note="FAM-CGCACCGATTATTTATACTTTTAGTCCACCT-TAMRA"
FT   primer_bind     complement(68..88)
FT                   /standard_name="Primer reverse: MDB694"
FT                   /note="TGCTAACGGGTGCATCGTCTA"
FT                   /target="3'-host genome"
XX
SQ   Sequence 88 BP; 22 A; 20 C; 20 G; 26 T; 0 other;
     agctggcgta atagcgaaga ggcccgcacc gattatttat acttttagtc cacctggttt        60
     ttattgatag acgatgcacc cgttagca                                           88
//