ID   QT-EVE-ZM-017; SV 0; linear; genomic DNA; STS; SYN; 111 BP.
XX
AC   REN-00038-3;
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DT   09-MAR-2006
DT   27-OCT-2010
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DE   Quantitative PCR method for detection of maize event LY038
DE   (Charles Delobel et al., 2008). 
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KW   event_specific.
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OS   Zea mays (maize) - event LY038 (REN-00038-3)
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RN   [1]
RP   1-111
RA   Charles Delobel C., Grazioli E., Larcher S., Mazzara M., Van Den Eede G.;
RT   "Event-specific Method for the Quantification of Maize Line LY038 Using Real-time PCR - Validation Report and Protocol";
RL   Online Publication (2008).
RX   DOI=10.2788/43570
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RN   [2]
RP   1-111
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2010).
RX   PCR=QT-EVE-ZM-017.pdf
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DR   GMOMETHODS; QT-TAX-ZM-002;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..111
FT                   /standard_name="PCR 111 bp amplicon"
FT                   /note="event-specific RT-PCR"
FT                   /target="5' integration border region (IBR) between the insert of maize event LYO38 and the maize host genome"
FT   primer_bind     1..21
FT                   /standard_name="Primer forward: LY038 AF"
FT                   /note="TGGGTTCAGTCTGCGAATGTT"
FT                   /target="5'-host genome"
FT   primer_bind     61..84
FT                   /standard_name="RT-PCR probe: LY038 AP"
FT                   /note="FAM-CGAGCGGAGTTTATGGGTCGACGG-TAMRA"
FT   primer_bind     complement(87..111)
FT                   /standard_name="Primer reverse: LY038 AR"
FT                   /note="AGGAATTCGATATCAAGCTTATCGA"
FT                   /target="insert"
XX
SQ   Sequence 111 BP; 22 A; 22 C; 37 G; 30 T; 0 other;
     tgggttcagt ctgcgaatgt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn        60
     cgagcggagt ttatgggtcg acggnntcga taagcttgat atcgaattcc t                111
//