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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)


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ID   QT-TAX-GM-004; SV 0; linear; genomic DNA; STS; PLN; 70 BP.
XX
AC   ;
XX
DT   29-MAY-2009
DT   01-OCT-2010
XX
DE   Quantitative PCR method for detection of soybean lectin gene
DE   (Hird et al., 2003).
XX
KW   taxon_specific, validated_in_combination.
XX
OS   Glycine max (soybean)
XX
RN   [1]
RP   1-70
RA   Hird H., Powell J., Johnson M.L., Oehlschlager S.;
RT   "Determination of percentage of RoundUp Ready soya in soya flour using
RT   real-time polymerase chain reaction: interlaboratory study";
RL   J AOAC Int 86:66-71 (2003).
RX   PUBMED; 12607742.
XX
RN   [2]
RP   1-70
RT   "See Cross-references below";
RL   Online Publication (2010).
XX
DR   GMOMETHODS; QT-CON-00-002;
XX
FH   Key             Location/Qualifiers
FH
FT   STS             1..70
FT                   /standard_name="PCR 70 bp amplicon"
FT                   /note="Taxon-specific duplex RT-PCR"
FT                   /target="lectin (Le1) gene"
FT   primer_bind     1..18
FT                   /standard_name="Primer forward: Soya lectin-F"
FT                   /note="TGGTCGCGCCCTCTACTC"
FT                   /target="Le1"
FT   primer_bind     complement(26..54)
FT                   /standard_name="RT-PCR probe: Soya lectin"
FT                   /note="VIC-CTACCGGTTTCTTTGTCCCAAATGTGGAT-TAMRA"
FT   primer_bind     complement(55..70)
FT                   /standard_name="Primer reverse: Soya lectin-R"
FT                   /note="GGCGAAGCTGGCAACG"
FT                   /target="Le1"
XX
SQ   Sequence 70 BP; 14 A; 27 C; 15 G; 14 T; 0 other;
     tggtcgcgcc ctctactcnn nnnnnatcca catttgggac aaagaaaccg gtagcgttgc        60
     cagcttcgcc                                                               70
//