Only one hit for query 'ac%3aSYN-IR102-7'
ID QL-EVE-EC-001; SV 0; linear; unassigned DNA; STS; SYN; 90 BP.
XX
AC ;
XX
DT 23-JUN-2008
DT 23-NOV-2017
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DE Qualitative PCR method for detection of E. coli K-12 event AG3139 (Mazzara et al., 2009).
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KW event_specific.
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OS Escherichia coli K-12 (bacteria) - event AG3139
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RN [1]
RP 1-90
RA Mazzara M., Foti N., Savini C., Bonfini L., Van den Eede G.;
RT "Event-specific method for the detection of dried-killed bacterial biomass PT73 (TM) derived from E. coli GM strain AG3139 using real-time PCR - Validation Report and Protocol";
RL Online Publication (2009).
RX DOI=10.2788/58900.
XX
RN [2]
RP 1-90
RT "PCR reactions set up and amplification conditions";
RL Online Publication (2017).
RX PCR=QL-EVE-EC-001.pdf
XX
FH Key Location/Qualifiers
FH
FT STS 1..90
FT /standard_name="PCR 90 bp amplicon"
FT /note="Event-specific RT-PCR";
FT /target="5' integration border region (IBR) between the insert of E. coli K-12 event AG3139 and the bacterial host genome";
FT primer_bind 1..30
FT /standard_name="Primer forward: TMD-For"
FT /note="AATACCGTTAAACGTAAATTCTTTTTCTTT"
FT /target="5'-host genome"
FT primer_bind 33..68
FT /standard_name="RT-PCR probe: TMD-probe"
FT /note="FAM-AGATCGAGTATTCATTCGGTGTATTGATTCACTTGA-TAMRA"
FT primer_bind complement(71..90)
FT /standard_name="Primer reverse: TMD-Rev"
FT /note="TCCTCCCGGTTTTTTTCGTA"
FT /target="insert"
XX
SQ Sequence 90 BP; 30 A; 13 C; 17 G; 30 T; 0 other;
aataccgtta aacgtaaatt ctttttcttt nnagatcgag tattcattcg gtgtattgat 60
tcacttgann tacgaaaaaa accgggagga 90
//