Only one hit for query 'ac%3AMST-FG072-2'
ID QL-CON-00-014; SV 1; linear; genomic DNA; STD; SYN; 170 BP.
XX
AC ;
XX
DT 22-DEC-2008
DT 08-JUN-2017
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DE Qualitative PCR method for detection of the junction between the nos promoter and the neomycin phosphotransferase II gene
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KW construct_specific.
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OS Carica papaya (papaya) - event Sunup-Papaya 55-1
XX
RN [1]
RP 1-170
RT "Horizontal methods for molecular biomarker analysis -- Methods of analysis for the detection of genetically modified organisms and derived products - Part 4: Real-time PCR based screening methods for the detection of the P-nos and P-nos-nptII DNA sequences";
RL ISO/TS 21569-4:2016 (2016).
RX ISO=69339
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RN [2]
RP 1-170
RT "Detection of a DNA sequence junction between the nos-promoter and the nptII-gene for screening of genetically modified organism using real-time PCR";
RL BVL L 00.00-142:2013-01 Food Analysis, Beuth, Berlin Koln (2013).
RX BVL=bvl-l-00-00-142/179718833
XX
RN [3]
RP 1-170
RT "PCR reactions set up and amplification conditions";
RL Online Publication (2017).
RX PCR=QL-CON-00-014.pdf
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FH Key Location/Qualifiers
FH
FT STS 1..170
FT /standard_name="PCR 144-170 bp amplicon"
FT /note="Construct-specific RT-PCR"
FT /target="Junction region between the nopaline synthase promoter (P-nos) from Agrobacterium tumefaciens and the neomycin phosphotransferase II (nptII) gene"
FT misc_feature 1..170
FT /note="The fragment size generated by the P-nos-nptII PCR depends on the assembly of the two sequence elements and is varying in the different GM plant events"
FT primer_bind 1..22
FT /standard_name="Primer reverse: nptII-R"
FT /note="GATTGTCTGTTGTGCCCAGTCA"
FT /target="nptII gene"
FT primer_bind 24..48
FT /standard_name="RT-PCR probe: nptII-Tm2"
FT /note="FAM-AGCCGAATAGCCTCTCCACCCAAGC-BBQ"
FT primer_bind complement(148..170)
FT /standard_name="Primer forward: pnos-F2"
FT /note="TTCCCCTCGGTATCCAATTAGAG"
FT /target="P-nos"
XX
SQ Sequence 170 BP; 47 A; 34 C; 50 G; 39 T; 0 other;
gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc 60
tgcccggatc cgggcggaaa taggtaaaga agttgcggat aaggtaattg ccattgcaga 120
ttatttggat tgagagtgaa tatgagactc taattggata ccgaggggaa 170
//