ID   QT-EVE-ZM-011; SV 0; linear; genomic DNA; STS; SYN; 102 BP.
XX
AC   ACS-ZM003-2;
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DT   20-NOV-2009
DT   12-FEB-2015
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DE   Quantitative PCR method for detection of maize event T25
DE   (Mazzara et al., 2013).
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KW   event_specific.
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OS   Zea mays (maize) - Event T25 (ACS-ZM003-2)
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RN   [1]
RP   1-102
RA   Mazzara M., Grazioli E., Savini C., Van Den Eede G.;
RT   "Event-specific Method for the Quantification of Maize Line T25 Using Real-time PCR v. 1.01 - Validation Report and Validated Method";
RL   Online Publication (2013).
RX   DOI=10.2788/18420
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RN   [2]
RP   1-102
RT   "PCR reactions set up and amplification conditions";
RL   Online Publication (2013).
RX   PCR=QT-EVE-ZM-011.pdf
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DR   GMOMETHODS; QT-TAX-ZM-003;
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FH   Key             Location/Qualifiers
FH
FT   STS             1..102
FT                   /standard_name="PCR 102 bp amplicon"
FT                   /note="event-specific RT-PCR"
FT                   /target="3' integration border region (IBR) between the insert of maize event T25 and the maize host genome"
FT   primer_bind     1..20
FT                   /standard_name="Primer forward: MLD143"
FT                   /note="ACAAGCGTGTCGTGCTCCAC"
FT                   /target="insert"
FT   primer_bind     46..70
FT                   /standard_name="RT-PCR probe: TM016"
FT                   /note="FAM-TCATTGAGTCGTTCCGCCATTGTCG-TAMRA"
FT   primer_bind     complement(82..102)
FT                   /standard_name="Primer reverse: MDB551"
FT                   /note="GACATGATACTCCTTCCACCG"
FT                   /target="3'-host genome"
XX
SQ   Sequence 102 BP; 18 A; 25 C; 28 G; 31 T; 0 other;
     acaagcgtgt cgtgctccac nnnnnnnnnn nnnnnnnnnn nnnnntcatt gagtcgttcc        60
     gccattgtcg nnnnnnnnnn ncggtggaag gagtatcatg tc                          102
//